Automation of Liquid Nitrogen Freezer for Cryopreservation of Unique Human Biospecimens at the Vanderbilt Biospecimen Storage Facility

项目摘要 与生物储存库相关联的大型、高维临床、遗传和生物标志物数据正在推动基础、临床和生物医学的发展。 和翻译研究。在过去的20年里，范德比尔特大学医学中心（VUMC） 对转化科学进行长期战略投资，作为临床和基础研究领域的卓越平台 科学部门，并被国际公认为个性化医疗等领域的领导者， 疫苗学和癌症护理和研究。例如，BioVU，范德比尔特的大型生物储存库， 包含与去识别丢弃样本相关的详细去识别人类表型信息， 基因型冷冻保存的人体样本使范德比尔特疫苗中心能够分离出 针对COVID-19的单克隆抗体被开发成预防COVID-19的疗法Evushold 在高危人群中。VUMC的合作人体组织网络是由国家资助的仅有的六个之一。 癌症研究所采购和分发人体组织的研究人员在美国，加拿大， 化国际大这些资源和其他资源提供了一个加速发现的引擎。统称 这些努力提供了疾病风险、疾病进展或对治疗的反应的有力预测指标， 支持研究描绘疾病的基本机制以及翻译到临床。 这些大规模的举措产生了大量有价值的人类和动物组织样本。这些 组织只有在储存在液氮中时才能有效地冷冻保存。这些生物储存库的大小 超出了单个研究者或团队有效管理、保持样本完整性的能力， 快速提取样本进行进一步研究。这些材料中有许多是不可替代的， 国际重要性。为了满足这一巨大且不断增长的需求，专门的自动化-190 ° C低温 冷冻样品存储系统是必要的管理，存储和检索数以万计的 生物储藏室样品。该提案旨在获得购买Azenta BioStore Cryo M60的资金 机器人液氮冷冻系统，可以容纳所有大型生物储存库在范德比尔特。有了这样的 系统范德比尔特可以提供必要的机构支持，环境和专业知识，以确保成本- 这些关键样品和试剂的有效、有质量保证的管理、储存和检索。的 技术是持久的和可扩展的，因此可以预测，这种自动化系统将使 广泛的研究人员，并推动基础，临床和转化研究长期到未来。

Project Summary Large, high-dimensional clinical, genetic and biomarker data linked to bio-repositories are driving basic, clinical and translational research. For the past 20 years, Vanderbilt University Medical Center (VUMC) has made long-term strategic investments in translational sciences as a platform for excellence across clinical and basic science departments and is internationally recognized as a leader in such fields as personalized medicine, vaccinology, and cancer care and research. As examples, BioVU, Vanderbilt’s large-scale biorepository, contains detailed de-identified human phenotype information linked to de-identified discarded samples and genotypes. Viably cryopreserved human specimens enabled the Vanderbilt Vaccine Center to isolate monoclonal antibodies against COVID-19 that were developed into Evusheld, a therapy to prevent COVID-19 in at-risk individuals. Cooperative Human Tissue Network at VUMC is one of only six funded by the National Cancer Institute to procure and distribute human tissues to researchers throughout the US, Canada, and internationally. These resources and others provide an accelerating engine of discovery. Collectively these endeavors are providing powerful predictors of disease risk, disease progression or response to therapy and underpinning studies delineating the fundamental mechanism of disease as well as translation to the clinic. These large-scale initiatives generate a massive number of valuable human and animal tissue samples. These tissues can only be viably cryopreserved when stored in liquid nitrogen. The size of these biorepositories is exceeding the capacity of individual investigators or groups to effectively curate, maintain sample integrity and rapidly retrieve samples for further study. Many of these materials could not be replaced and are of international importance. To meet this large and growing need, a specialized automated -190°C Cryogenic Freezer for Sample Storage system is necessary to manage, store and retrieve the tens of thousands of biorepository samples. This proposal seeks to obtain funding for the purchase of an Azenta BioStore Cryo M60 robotic liquid nitrogen freezer system that can accommodate all large biorepositories at Vanderbilt. With such a system Vanderbilt can provide the necessary institutional support, environment and expertise to ensure cost- effective, quality assured management, storage and retrieval of these critical samples and reagents. The technology is durable and scalable and therefore it can be predicted that this automated system will enable a broad range of investigators and propel basic, clinical and translational research long into the future.