TAKASHIMA DIVERSITY SUPPLEMENT TO: REGULATION OF GAMETE USE AND NEURAL PATHWAYS IN REPRODUCTION

项目摘要 这是一个多元化的补充，以支持高岛洋子的职业发展， 从事R 01 HD 059060“法规”相关工作的二年级博士生 生殖中的配子使用和神经通路。她的工作集中在三个主要目标上 与繁殖成功的决定因素有关。第一个目的是阐明功能 交配期间外泌体（囊泡）从雄性转移到雌性的后果， 这种现象在哺乳动物和果蝇中也能看到。这些转移的外泌体的功能， 包裹和转移蛋白质和RNA的微小脂质结合囊泡， 在任何系统中都可以理解。在果蝇中，它们被认为会影响 精子竞争，连续雄性的射精竞争受精 机会Takashima女士将评估外泌体产生，转移及其 影响精子竞争，使她能够应用全基因组关联（GWAS） 方法来识别以前未知的影响外泌体生物学的遗传变异。在她 第二个目标，Takashima女士将剖析外泌体内容所采用的机制， 影响雌性交配后的反应，包括精子竞争能力的特定测定。 最后，在目标3中，这些数据将被纳入一个有前途的统计建模方法， Bradley-Terry模型，以量化扰动的精液外泌体对男性的影响， 竞争繁殖成功我们制定了详细的指导计划， 女士高岛为她的科学发展提供了量身定制的支持， 她的职业目标她将接受学术和研究培训， 研究技能和负责任的行为，教学/指导经验，并指导 完成一个有影响力的博士学位她还将获得额外的指导，以帮助她选择 她正在考虑的职业选择;这将涉及她的导师一对一的指导， 我们将为她建立与同事和校友的网络，她正在探索的职业， 以及她将参加的康奈尔大学和会议的研讨会和资源。

Project Summary This is a Diversity Supplement to support the development of the career of Yoko Takashima, a second year PhD student who is engaged in work related to R01 HD059060, “Regulation of Gamete Use and Neural Pathways in Reproduction”. Her work focuses on three primary aims related to the determinants of reproductive success. The first aim is to elucidate the functional consequences of the transfer of exosomes (vesicles) from males to females during mating, a phenomenon seen in mammals and in Drosophila. The function of these transferred exosomes, minute lipid-bound vesicles that encapsulate and transfer proteins and RNAs, is not well- understood in any system. In Drosophila they have been suggested to affect the process of sperm competition, in which ejaculates from successive males compete for fertilization opportunities. Ms. Takashima will assess the variation in exosome production, transfer, and its effects on sperm competition, allowing her to apply genome-wide association (GWAS) approaches to identify previously unknown genetic variants that impact exosome biology. In her second aim, Ms. Takashima will dissect the mechanism(s) employed by exosome contents to impact female post-mating responses, including a specific assay of sperm competitive ability. Finally, in Aim 3, these data will be incorporated into a promising statistical modeling approach, the Bradley-Terry model, to quantify the effects of perturbed seminal exosomes on male competitive-reproductive success. We have devised a detailed mentoring plan that will provide Ms. Takashima tailored support for both her scientific development and to guide her in achieving her career goals. She will receive academic and research training, training in presentational skills and responsible conduct in research, teaching/mentoring experience, and guidance to complete an impactful PhD. She will also receive additional guidance to help her choose among career options that she is considering; this will involve one-on-one guidance from her mentors, networking that we will set up for her with colleagues and alumni in careers she is exploring, as well as workshops and resources at Cornell and at conferences she will attend.