Identification of the 1-methyladenine receptor

这项拟议的研究的目标是识别和表征卵巢中卵母细胞成熟激素受体。 海星，Patina mini ata。与其他模型系统相比，该模型系统具有显著的优势，在某些方面可以 与哺乳动物卵母细胞共享。这项拟议的工作旨在识别和表征这种受体 使用独特的基于同源性的方法和基于功能表达克隆的方法相结合来识别和 编码成熟诱导激素受体1-甲基腺嘌呤(1-甲基腺嘌呤)的候选基因克隆(S) Ma)。使用这些新策略成功识别1-MA受体将是第一次 明确描述任何物种中的成熟诱导激素受体，并提供概念证明 该策略将用于鉴定其他物种的成熟激素受体，包括 哺乳动物。几个候选DNA已经被分离出来；这些和其他候选DNA将被 将接受进一步的功能研究以确认身份，并测试卵母细胞成熟的必要性 指挥。最终，将进行旨在阐明分子细节的研究 卵母细胞成熟过程中的信号传导机制。

The goal of the proposed study is to identify and characterize the oocyte maturation hormone receptor in the starfish, Patina miniata. This model system offers signficant advantages over others and some aspects may be shared with mammalian oocytes. The proposed work is designed to identify and characterize this receptor using unique homology based and functional expression cloning based approaches in tandem to identify and clone candidate cDNA(s) encoding the receptor for the maturation-inducing hormone, 1-methyladenine (1- MA). Successful identification of the 1-MA receptor using these novel strategies would represent the first definitive description of a maturation-inducing hormone receptor in any species and provide proof of concept for the strategy to be used for the identification of maturation hormone receptors in other species, including mammals. Several candidate cDNAs already have been isolated; these and additional candidates will be subjected to further functional studies to confirm identity and tests for necessity in oocyte maturation will be conducted. Eventually, studies will be conducted that are designed to elucidate the details of the molecular mechanism of signal transduction during oocyte maturation.