Role of BMP4 in hESC derived endothelial progenitors

胚胎干细胞(HESC)可能被用作血管发育的模型， 血管生成、血管发病机制、药物发现和组织工程。然而，人们对此知之甚少 控制人胚胎干细胞向内皮细胞分化的机制。为 例如，骨形态发生蛋白(BMP)4是一种有效的中胚层诱导剂，已知在血管中发挥作用。 BMP4在各种动物模型中的发展，但尚不清楚BMP4在人类血管生成中扮演什么角色。 在这里，我们发展了一个基于类胚体附着在Matrigel上的hESC分化模型 凝胶底物在EGM-2培养基中。培养7天后，RT-PCR检测到的细胞数量显著增加 内皮标志物(如KDR、VE-Cad、PECAM1、CD34、eNOS、vWF、Tie-2)的表达。我们的 初步结果表明，可以使用相对简单的hESC发育血管生成模型。 为了研究时间基因的表达，我们建议研究BMP4在hESC中的作用 分化与内皮细胞发育。本研究中使用的细胞系的NIH hESC注册码 它们是：BG01、BG02和WA09。

an embryonic stem cells (hESC) can potentially be used as models of vascular development, angiogenesis, vascular pathogenesis, drug discovery and tissue engineering. Yet little is known about the mechanisms controlling hESC differentiation along the pathway toward the endothelial lineage. For example, bone morphogenic protein (BMP) 4, a potent mesoderm inducer, is known to play a role in vascular development in various animal models but it is unclear what role BMP4 plays in human vasculogenesis. Here we have developed a model of hESC differentiation based on embryoid bodies adhering to a Matrigel gel substrate in EGM-2 medium. After 7 days in culture we detected by RT-PCR significantly increased expression of endothelial markers (e.g. KDR, VE-Cad, PECAM1, CD34, eNOS, vWF, Tie-2). Our preliminary results indicate that a relatively simple hESC developmental vasculogenesis model may be used to study temporal gene expression thereforewe propose to examine the role of BMP4 in the hESC differentiation and endothelial development. The NIH hESC registry codes for cell lines used in this research are:BG01,BG02andWA09.