Imaging spatial transcriptome at single cell resolution to study eye regeneration in old age

摘要 老年疾病的治疗对老年人的生活质量至关重要。一个很有希望的战略 就是用再生医学延缓衰老或逆转衰老。然而，茎的再生能力 细胞及其支持的微环境在老年时会受到严重损害。因此，要恢复功能 老年成体干细胞及其微环境或维持成体干细胞年轻功能 老年是赋予再生医学抗击与年龄相关疾病的能力的关键研究方向。这里, 我们建议使用一种尖端的空间转录方法，序列范围，来全面研究 有性浮游动物眼祖细胞及其壁龛细胞的衰老、再生和复壮 组织再生的冠军。我们的目标是确定促进再生和 晚年返老还童。拟议的实验将定义具有根本重要性的数据集 识别利基细胞以及老化和再生的变化，这将推动长期的机制研究。

ABSTRACT To cure age-related diseases is of critical importance for the life quality of elderly people. One promising strategy is to slow down aging or reverse aging with regenerative medicine. However, the regenerative capacity of stem cells and their supportive microenvironments are drastically impaired in old age. Hence, to rejuvenate functions of aged adult stem cells and their microenvironments or to maintain the youthful functions of adult stem cells in old age are critical research directions to empower regenerative medicine to fight age-associated diseases. Here, we propose to use a cutting-edge spatial transcriptomics method, Seq-Scope, to comprehensively examine the aging, regeneration and rejuvenation of eye progenitor cells and their niche cells in the sexual planarians, a champion of tissue regeneration. We aim to determine the molecular pathways that facilitate regeneration and rejuvenation in old age. The proposed experiments will define datasets of fundamental importance in the identification of niche cells and changes in aging and regeneration that will fuel long-term mechanistic studies.