Extracellular Potassium and Astrocytic Kir4.1 Modulate Interneuron Activity in the Cerebral Cortex

项目总结 本申请是对R01 NS127819-01“胞外钾和 星形细胞KIR4.1调节大脑皮层的中间神经元活性“，Moritz博士 塔夫茨大学的阿姆布鲁斯特。这位候选人是雷纳·加里佩，他是斯坦福大学实验室的博士前学生。 阿姆布鲁斯特。雷纳将使用实验室中目前使用的工具，并使用它们来追求她的兴趣，以进行调查 星形胶质细胞如何塑造GABA动力学。雷纳作为拉丁裔候选人有资格成为多元化候选人，首先 一代大学生，以及她的经济贫困背景。因此，她是 多个代表不足的群体。这份附录将为雷纳三年的毕业论文工作提供资金。 第三年的资金将取决于第二年期间提交的NRSA申请。在这段时间里，雷纳将 接受细胞神经科学、神经胶质生物学、癫痫等方面的培训。她将在以下方面获得专业知识 生化分析，星形胶质细胞和神经元电生理学，iGABASnFR成像，以及数据和统计 分析。这项培训将引导她申请NRSA，并成为神经科学领域的领导者 研究。加里皮致力于学术研究，重点是癫痫的发生。阿姆布鲁斯特博士，是一位 塔夫茨大学神经科学系初级研究人员，具有丰富的 星形胶质细胞-神经元相互作用和利用活细胞成像方法。为了增加雷纳的机会 为了促进发展，我们邀请了Chris Dulla博士作为高级导师。杜拉博士有很好的记录 导师关系，包括2018年兰迪斯杰出导师奖。这里列出的项目将提供 Gariepy女士在GABA神经传递、神经元/神经胶质相互作用和切割方面接受了出色而严格的培训- 边缘成像设备。ArmBruster博士和Gariepy女士已经制定了一种有条理的、严格的和 定制辅导和培训计划。通过这一补充提供的资金将给雷纳一个明确的 作为癫痫和GABA领域的一名有前途的研究人员，通向令人兴奋的未来之路 神经传递。所安排的活动和丰富的机会对Gariepy女士的 作为一名成功的独立调查员的发展和未来。

PROJECT SUMMARY This application is for a diversity supplement to the R01 NS127819-01 “EXTRACELLULAR POTASSIUM AND ASTROCYTIC KIR4.1 MODULATE INTERNEURON ACTIVTY IN THE CEREBRAL CORTEX” of Dr. Moritz Armbruster at Tufts University. The candidate is Reyna Gariepy, a pre-doctoral student in the lab of Dr. Armbruster. Reyna will take tools currently in use in the lab and use them to pursue her interests to investigate how GABA dynamics are shaped by astrocytes. Reyna qualifies as a diversity candidate as a Latina, first generation college student, and her economically disadvantaged background. Therefore, she is a member of multiple underrepresented groups. This supplement will fund Reyna for 3 years of her graduate thesis work. Funding for Y3 will be contingent on submission of an NRSA application during Y2. During this time Reyna will receive training in cellular neuroscience, glial biology, epilepsy and more. She will gain expertise in biochemical assays, astrocytes and neuronal electrophysiology, iGABASnFR imaging, and data and statistical analysis. This training will guide her towards an NRSA application and future as a leader in neuroscience research. Ms. Gariepy is devoted to academic research with a focus on epileptogenesis. Dr. Armbruster, is a junior research faculty in the Neuroscience Department at Tufts University who has extensive experience in astrocyte-neuron interactions and utilizing live-cell imaging approaches. To increase Reyna’s opportunities for development, we have included Dr. Chris Dulla as a senior mentor. Dr. Dulla has a great track record of mentorship, including the 2018 Landis Award for Outstanding Mentorship. The project laid out here will give Ms. Gariepy excellent and rigorous training in GABA neurotransmission, neuron/glial interactions, and cutting- edge imaging modalities. Dr. Armbruster, and Ms. Gariepy have developed a structured, rigorous, and customized mentorship and training plan. The funding offered through this supplement will give Reyna a clear path toward an exciting future as an up and coming researcher in the field of epilepsy and GABA neurotransmission. The activities laid out and the enrichment opportunities will be critical to Ms. Gariepy’s development and future as a successful independent investigator.