Detection of Anthrax Infection by Magnetic Reporting of Molecular Sensors

塔夫茨大学医学院 爱德华·戈德堡博士正在设计分子传感器，这种传感器将使用磁性报告来检测炭疽病 感染。长期目标是开发一种手持诊断设备，它将使用非破坏性的 患者样本中炭疽病的检测方法。他们正在使用噬菌体尾部纤维蛋白 测定用作检测生物传感器的蛋白质-纳米颗粒结合物的磁化率。他 要求生产感染了长尾纤维突变形式的噬菌体的大肠杆菌细胞颗粒 T4.)。用噬菌体感染CO//培养物。因为我们使用发酵罐和 可编程控制系统我们能够为快速生长的培养物提供充足的氧气供应， 戈德堡博士之前在自己的实验室里所做的努力中遇到的问题。我们完成了一个 发酵过程产生180克细胞颗粒，在戈德堡博士的实验室进行进一步处理。

Tufts University Medical School Dr. Edward Goldberg is designing molecular sensors that will use magnetic reporting to detect anthrax infection. The long-term goal is to develop a hand-held diagnostic device that will use a non-destructive detection method to identify anthrax in patient samples. They are using bacteriophage tail fiber protein to determine the magnetic susceptibility of protein-nanoparticle conjugates for use as detection biosensors. He requested the production of E. coli cell pellets infected with a mutant form of the long tail fiber of bacteriophage T4. ¿. co// cultures were grown and infected with bacteriophage. Because we use fermentors with a programmable control system we were able to provide the fast growing culture with sufficient oxygen supply, a problem Dr. Goldberg had encountered in his previous efforts in his own laboratory. We completed one fermentation run that generated 180 g of cell pellet which was further processed in Dr. Goldberg's laboratory.