THE EFFECT OF ISOFLURANE ON TRANSCAPILLARY FLUID REDISTRIBUTION AFTER HEMORRHAGE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 背景： 液体治疗很重要，尤其是对病情严重的患者，而且对于补充手术中丢失的液体和其他元素也很重要。 之前的绵羊研究表明，麻醉气体促进了血管外液体的积累。此外，在绵羊模型中，麻醉气体已被证明可以削弱毛细血管对液体丢失的正常补充反应。 具体目标和程序： 该项目的目标是确定麻醉气体是否减少了尿液的产生，并导致受试者在受控出血期间将液体分配到血管外。 实验设计(摘要)： 为了实现这些目标，我们将招募12名志愿者参加我们的流体动力学研究。体液移位将使用一种被称为指示器稀释技术的常用方法来计算，以评估基线容量状态。在测量基线后，将定期收集血红蛋白值。每个参与者将在为期六个月的时间里参加4个ARM的研究。这项研究的4个分支的描述如下： 1.清醒，抽血，不输液(仅清醒) 2.麻醉、抽血、不输液(仅限睡眠) 3.清醒、抽血、补液(清醒加输液) 4.麻醉、抽血、输液(睡眠加输液) 意义(摘要)： 这项研究的重要性在于能够更好地管理常见的临床事件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Fluid therapy is important, especially in very sick patients, and also important for the replacement of fluids and other elements lost during surgery. Previous sheep studies have shown that anesthesia gases promote the accumulation of fluid outside of blood vessels. Furthermore, in sheep models, anesthesia gases have been shown to blunt the refilling response that capillaries normally have to fluid loss. Specific Aims and Procedures: The goal of this project is to determine if anesthesia gases decrease the production of urine and cause fluid to be allocated outside the blood vessels in human participants during a controlled bleed. Experimental Design (summary): To accomplish these goals, we will enroll 12 volunteers into our fluid kinetics study. Body fluid shifts will be calculated using a commonly used method known as the indicator dilution technique to asses the baseline volume status. After the baseline is measured, hemoglobin values will be collected at regular intervals. Each participant will take part in 4 arms of the study over a period of six months. The description of the 4 arms of the study is as follows: 1. conscious, blood withdrawal, no fluid infusion (awake only) 2. anesthetized, blood withdrawal, no fluid infusion (asleep only) 3. conscious, blood withdrawal, fluid infusion (awake plus infusion) 4. anesthetized, blood withdrawal, fluid infusion (asleep plus infusion) Significance (summary): The importance of this study is the ability to better manage a common clinical occurrence.