GENOTYPIC INFLUENCES ON PREFRONTALLY-GUIDED BEHAVIORS IN ADOLESCENTS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 该项目将研究基因型对9至25岁的健康青少年和年轻人的前额叶引导行为的影响。神经行为发展中心正在进行一项行为研究，参与者将进行4-6小时的神经认知研究。大多数实施的措施都是经过精心挑选的，以招募前额叶皮层背侧、内侧和腹侧区域的活动。识别记忆和运动功能的不同措施也包括在内，因为是个性的措施。人类分子遗传学文献表明，许多这些功能（额叶介导的行为，人格特质）受到影响多巴胺能和多巴胺能神经传递的基因中的基因型变异的影响。在这项研究中，我们希望检查三个基因（儿茶酚-O-甲基转移酶，多巴胺D4受体，5-羟色胺转运蛋白）的功能多态性与青少年的前额叶功能。我们将考虑基因型对跨任务行为表现的影响，以及在每个年龄和成熟组内，以确定基因型-认知相互作用是否在青春期前与青春期后同样有效。这项工作将对精神病理学的研究产生深远的影响，因为许多遗传性疾病（如精神分裂症，药物滥用）在青春期表现出来。要求GCRC的护理和实验室服务（a）抽取血液样本，（B）处理样本进行分子遗传学分析，（c）DNA提取，以及（d）基因分型沿着所述的三个维度。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project will examine genotypic influences on prefrontally-guided behaviors in healthy adolescents and young adults, ages 9 to 25 years. A behavioral study is underway in the Center for Neurobehavioral Development whereby participants unergo a 4-6 hour neurocognitive study. Most of the measures implemented have been carefully selected to recruit activity in the dorsal, medial, and ventral regions of the prefrontal cortex. Diverent measureds of recognition memory and motor function are also included, as are personality measures. The human molecular genetics literature suggests that many of these functions (frontal lobe mediated behaviors, personality traits) are impacted by genotypic variants in genes that influence dopaminergic and serotoninergic neurotransmission. In this study, we wish to examine functional polymorphisms of three genes (the catechol-o-methyltransferase, dopamine D4 receptor, and serotonin transporter) in relation to prefrontal function in adolescents. We will consider the impact of genotype on behavioral performance across tasks as well as within each age and maturational group to determine whether genotype-cognition interactions are equally potent pre-versus post-puberty. This work will have profound implications for the study of psychopathology, since many genetically-based disorders (e.g. schizophrenia, substance abuse) manifest during adolescence. The GCRC's nursing and laboratory services are requested to (a) draw blood samples, (b) process the samples for molecular genetic analysis, (c) DNA extraction, and (d) genotyping along the three dimensions to be described.