SIGNAL TRANSDUCTION CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该核心的目的是为有兴趣研究细胞转导和信号通路的研究人员提供设计，制造表达荧光融合蛋白的载体以用于细胞内定位和蛋白质运输的技术援助。荧光标记蛋白质研究的观察结果通常显示蛋白质的稳态分布，但它们并不直接提供分子动力学特性的信息。购买了尼康单分子成像系统，该系统配备了在37摄氏度孵育时对单细胞进行延时摄影，现在已完全配备了Metamorph软件，可用于活细胞成像研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this core is to provide technical assistance in designing, making vectors expressing fluorescent fusion proteins for intracellular localization and protein trafficking to investigators interested in studying cellular transduction and signaling pathways. Observations from studies with fluorescently tagged proteins typically show the steady state distribution of a protein, but they do not directly provide information about the kinetic properties of molecules. Acquisition of a Nikon single molecule imaging system equipped for time lapse photography of single cells while under incubation at 37 degrees C has been purchased and is now fully equipped with Metamorph software and functional for live cell imaging studies.