EFFECT OF A2-ANTIPLASMIN POLYMORPHISM ON CLOTTING MEASURES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是检测α 2-抗纤溶酶（α 2-AP）基因内单核苷酸多态性（SNP）的频率。 该SNP导致α 2-AP的长形式的位置6处从精氨酸（R）到色氨酸（W）的单个氨基酸变化。 我们的目的是确定SNP在一般人群中的频率，并确定这如何影响α 2-AP水平，蛋白质序列和体外凝血措施。将招募300名代表普通民众的捐助者。 预计这些捐助者中的大多数将是OUHSC的员工，学生或校园访客，并将通过在校园内张贴的广告招募。将要求受试者在上午8点至10点之间前往GCRC，并在抽血前禁食至少8小时。在获得知情同意后，测量并记录身高和体重，然后由医生、护士或经过培训的采血师采用无菌技术通过静脉穿刺抽取20 ml血液至ACD抗凝剂中。 抽血后，将为受试者提供小吃。 将从1 ml血液中分离DNA。PCR技术将用于扩增包含多态性的α 2-AP pro部分。 将通过PCR产物测序确定基因型。 将剩余的血液离心，收集血浆并冷冻，用于测定α 2-AP水平、蛋白质序列、α 2-AP原-裂解酶活性和用于体外凝血研究。本研究的目的是检测2-抗纤溶酶（2-AP）基因内单核苷酸多态性（SNP）的频率。 该SNP导致在2-AP的长形式的位置6处从精氨酸（R）到色氨酸（W）的单个氨基酸变化。 我们的目的是确定SNP在一般人群中的频率，并确定这如何影响α 2-AP水平，蛋白质序列和体外凝血措施。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this research project is to examine the frequency of a Single Nucleotide Polymorphism (SNP) within the gene for a2-antiplasmin (a2-AP). This SNP results in a single amino acid change from arginine (R) to tryptophan (W) at position 6 of the long form of a2-AP. Our aim is to determine the frequency of the SNP in the general population, and to determine how this affects a2-AP levels, protein sequence, and in vitro clotting measures. 300 donors representing the general population will be recruited. The majority of these donors is expected to be OUHSC employees, students, or visitors to campus and will be recruited with an advertisement posted on campus by Dr. Christiansen. The participants will be asked to come to the GCRC between 8 and 10 a.m. and be fasting for at least 8 hours before the blood draw. After obtaining informed consent, height and weight will be measured and recorded, then a physician, nurse or trained phlebotomist will utilize sterile technique to draw 20 ml of blood by venipuncture into ACD anticoagulant. Following the blood draw, a snack will be provided to participants. DNA will be isolated from 1 ml of the blood. PCR techniques will be used to amplify the portion of the a2-APpro encompassing the polymorphism. The genotype will be determined by sequencing of the PCR product. The remainder of the blood will be centrifuged and the plasma collected and frozen for determination of a2-AP levels, protein sequence, a2-APpro-cleaving enzyme activity and for in vitro clotting studies. Specific Aims The purpose of this research project is to examine the frequency of a Single Nucleotide Polymorphism (SNP) within the gene for 2-antiplasmin (2-AP). This SNP results in a single amino acid change from arginine (R) to tryptophan (W) at position 6 of the long form of 2-AP. Our aim is to determine the frequency of the SNP in the general population, and to determine how this affects a2-AP levels, protein sequence, and in vitro clotting measures.