ENDO-CELL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项建议涉及建立异种移植的人子宫内膜的功能模型。我们从18-45岁的女性志愿者身上收集子宫内膜活检组织，用于植入实验小鼠体内。其目标是研究已成功用于阐明啮齿动物子宫内膜中激素相互作用机制的药物或其他药物，但这些药物或药物实际上无法用于人类受试者。我们已经能够成功地将人子宫内膜植入小鼠肾被膜下，并在植入后4-6周显示组织增殖。目前，我们正在测试一种新的子宫内膜细胞周期调节途径，该途径最近在小鼠子宫内膜中得到了表征。如果成功，该项目可能会为开发雌激素诱导的癌前子宫生长的靶向抑制剂提供一个可能的跳板。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal involves creation of a functional model of xenografted human endometrium. We collect endometrial biopsy tissue from female volunteers ages 18-45 for implantation into laboratory mice. The goal is to study drugs or other agents that have been successfully used to elucidate mechanisms of hormonal interplay in the rodent endometrium but cannot be practically given to human subjects. We have been able to successfully implant human endometrium under the mouse renal capsule and show tissue proliferation 4-6 weeks after engraftment. Currently, we are testing a novel pathway of endometrial cell cycle regulation that has been recently characterized in the mouse endometrium. If successful, this project may provide with a possible springboard for development of targeted inhibitors of estrogen-induced pre-cancerous uterine growth.