NE COBRE: PROTEOMICS AND GENOMICS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这个核心的作用是在分子的测量和表征领域向病毒学中心的成员提供支持。质谱学部分专门致力于蛋白质组学领域的工作。蛋白质组学涉及使用质谱学来鉴定蛋白质，并确定在细胞生物学中对这些蛋白质进行的修饰。在我们的实验室中，我们将湿化学处理方法与层析、先进的质谱学技术和使用数据库挖掘的计算方法相结合，以识别可能参与与病毒相关的各种生物过程的蛋白质。这些研究使用一维SDS-PAGE和二维IEF-SDS-PAGE，然后在凝胶内胰酶消化和分离胰酶片段，结合电喷雾-四极杆飞行时间质谱仪。这些LC/MS和LC/MS/MS实验的结果在内部搜索已知蛋白质的专有和公共领域数据库。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The role of this core is to provide support to members of the Virology Center in the areas of measurement and characterization of molecules. The mass spectrometry component concentrates its efforts specifically on the area of proteomics. Proteomics involves using mass spectrometry for the identification of proteins and in determining modifications that are made to these proteins in the biology of the cell. In our laboratory we combine wet chemical processing methods with chromatography, advanced mass spectrometry techniques and computing methods using database mining to identify proteins which may be involved in the various biological processes associated with viruses. These studies were performed using both one dimensional SDS-PAGE and 2-dimensional IEF-SDS-PAGE, followed by in-gel trypsin digestion and separation of tryptic fragments with reverse phase HPLC coupled with electrospray -quadrapole-time-of-flight mass spectrometry. The results from these LC/MS and LC/MS/MS experiments were searched in-house against both proprietary and public domain databases of known proteins.