NE COBRE: PROTEOMICS AND GENOMICS CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。该核心的作用是在分子测量和表征领域为病毒学中心的成员提供支持。微阵列服务通过该核心在三个不同的设施向NCV成员提供，一个在UNL，两个在UNMC。在UNL的校园里，质谱分析部门将其工作重点集中在蛋白质组学领域。蛋白质组学包括使用质谱法鉴定蛋白质，并确定细胞生物学中对这些蛋白质的修饰。在我们的实验室，我们将湿化学处理方法与色谱法、先进的质谱技术和使用数据库挖掘的计算方法相结合，以识别可能参与与病毒相关的各种生物过程的蛋白质。这些研究采用一维SDS-PAGE和二维IEF-SDS-PAGE进行，随后采用凝胶内胰蛋白酶消化和反相高效液相色谱-电喷雾-四极柱-飞行时间质谱法分离胰蛋白酶片段。这些LC/MS和LC/MS/MS实验的结果在内部针对已知蛋白质的专有和公共数据库进行检索。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The role of this core is to provide support to members of the Virology Center in the areas of measurement and characterization of molecules. Micro array services are provided through this core to NCV members at three different facilities with one at UNL and two at UNMC. On the UNL campus, the mass spectrometry component concentrates its efforts specifically on the area of proteomics. Proteomics involves using mass spectrometry for the identification of proteins and in determining modifications that are made to these proteins in the biology of the cell. In our laboratory we combine wet chemical processing methods with chromatography, advanced mass spectrometry techniques and computing methods using database mining to identify proteins which may be involved in the various biological processes associated with viruses. These studies were performed using both one dimensional SDS-PAGE and 2-dimensional IEF-SDS-PAGE, followed by in-gel trypsin digestion and separation of tryptic fragments with reverse phase HPLC coupled with electrospray -quadrapole-time-of-flight mass spectrometry. The results from these LC/MS and LC/MS/MS experiments were searched in-house against both proprietary and public domain databases of known proteins.