Neutralization Core

核心C：免疫和中和核心 该核心将通过提供以下信息为HIVRAD的多个项目提供关键支持 天然和新型SIV和HIV-1包膜产生的中和抗体的效力和交叉反应 糖蛋白。我们已经组建了一支优秀的调查团队，他们结合了我们所有领域的专业知识 核心努力。我们的目标是与HIVRAD的设计和生产工作保持同步，及时提供 比较免疫原性数据。我们将在豚鼠和兔子身上进行非生殖研究，包括 使用标准佐剂和新型佐剂进行系统免疫和粘膜免疫。这些动物的样本将被评估 用于中和匹配的疫苗毒株和广泛的SIV或SIV异源毒株的抗体 酌情提供多种HIV-1基因亚型。此外，针对环境病毒的结合抗体将在 血清和阴道冲洗液中的免疫球蛋白和免疫球蛋白A类。这个核心C的长期目标是确定 免疫原、佐剂和免疫途径能够诱导中和抗体，这些抗体都是有效的。 对艾滋病疫苗的开发具有广泛的反应性。

Core C: Immunization and Neutralization Core This Core will provide critical support to multiple projects of this HIVRAD by providing information on the potency and cross-reactivity of neutralizing antibodies generated by native and novel SIV and HIV-1 envelope glycoproteins. We have assembled a team of outstanding investigators who have combined expertise in all areas of our Core effort. Our goal is to keep pace with the design and production efforts of this HIVRAD by providing timely comparative immunogenicity data. We will conduct irmamnogenicity studies in guinea pigs and rabbits that will include both systemic and mucosal immunity with standard and novel adjuvants. Samples from these animals wilt be assessed for antibodies that neutralize the matched vaccine strain and a wide spectrum of heterologous strains of either SIV or multiple genetic subtypes of HIV-1, as appropriate. In addition, Env-specific binding antibodies will be assessed in serum and vaginal lavages with respect to IgG and IgA classes. The long term goal of this Core C is to determine which immunogens, adjuvants and routes of immunization are capable of inducing neutralizing antibodies that are both potent and broadly-reactive for AIDS vaccine development.