Adherence Assessment for Supplements

描述（由申请人提供）：Caldera Pharmaceuticals使用蛋白质分级分离技术和XRpro技术测定蛋白质CAM/代谢物结合，作为测定NCCAM临床试验中CAM方案依从性的方法。Caldera将使用其高通量蛋白质组学技术，使用我们独特的、获得国家奖项的XRpro技术来识别CAM粘附生物标志物（CAB）。Caldera与洛斯阿拉莫斯国家实验室合作开发了XRpro。该技术允许通过测量由蛋白质-配体加合物和蛋白质-金属代谢物组成的CAB来鉴定和测量CAM粘附，而不需要先验知识来了解涉及哪些蛋白质。因此，不需要针对特定蛋白质的抗体或特定蛋白质测定。卡尔德拉制药公司有2000平方英尺的实验室空间和1800平方英尺的办公空间。该地区为化学和生物研发提供了良好的条件。可用于此项工作的设备包括：通风柜、灭菌烘箱、反应玻璃器皿、旋转蒸发器、反应和蒸馏真空管线、气氛控制手套箱、加热/搅拌罩、光谱仪（UV-VIS-NIR）、NMR、晶体学、荧光计、FTIR、TGA和DSC、显微镜，定制的EDAX Eagle III MXRF光谱仪和定制的EDAX Eagle II MXRF光谱仪和Horiba XGT 5000 XRF显微镜，以及定制的共焦XRF显微镜，FPLC，和Applied Biosystems Voyager-DE STR MALDI-TOF和QSTAR XL MS/MS质谱仪。Caldera可以进入机械和电子商店。Caldera设施符合联邦、州和地方政府关于以下方面的环境法律和法规：空气排放、水流出物、外部辐射水平、室外噪音、固体和散装废物处理实践以及有毒和有害材料的处理和储存。

DESCRIPTION (provided by applicant): Caldera Pharmaceuticals uses protein fractionation techniques and XRpro technology to determine protein CAM/metabolite binding as a means to determine adherence to CAM regimens in NCCAM clinical trials. Caldera will use their high-throughput proteomics technology to identify CAM Adherence Biomarkers (CABs) using our unique, national award winning XRpro technology. Caldera, in collaboration with Los Alamos National Laboratory, developed XRpro. This technology allows the identification and measurement of CAM adherence by measuring CABs consisting of protein-ligand adducts and protein-metal metabolites with no a priori knowledge of which proteins are involved. Therefore, antibodies to specific proteins or specific protein assays are not required. Caldera Pharmaceuticals has 2000 square feet of lab space and 1800 square feet of office space. This area is well equipped for chemical and biological R&D. Equipment available for this work includes: fume hoods, sterilization ovens, reaction glassware, rotary evaporator, vacuum lines for reactions and distillations, atmosphere controlled glove boxes, heating/stirring mantles, spectrometers (UV-VIS-NIR), NMR, crystallography, fluorimeters, FTIR, TGA and DSC, microscopes, a customized EDAX Eagle III MXRF spectrometer and a customized EDAX Eagle II MXRF spectrometer and Horiba XGT 5000 XRF microscope, and a custom-built confocal XRF microscope, FPLC, and an Applied Biosystems Voyager-DE STR MALDI-TOF and QSTAR XL MS/MS mass spectrometer. Caldera has access to machine and electronics shops. Caldera facilities meet environmental laws and regulations of federal, state, and local governments relating to: airborne emissions, waterborne effluents, external radiation levels, outdoor noise, solid and bulk waste disposal practices, and handling and storage of toxic and hazardous materials.