KCSA REVERSE MICELLE CB EXPERIMENT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 膜整合蛋白在神经动作电位、稳态、细胞结构和信号转导等生物学过程中发挥着重要作用。它们占目前和潜在药物靶点的50%以上，但它们的结构特征远远落后于可溶性蛋白。对于溶液核磁共振（NMR）光谱，膜蛋白是一个重大的挑战，因为它们在洗涤剂胶束中的溶解导致颗粒尺寸大，伴随着缓慢的分子取向。这就限制了光谱学的复杂性。 我们已经开发了一种新的程序，用于溶解膜蛋白在表面活性剂系统适合溶解在低粘度溶剂，如戊烷，丁烷，或丙烷，并在收集3D数据的过程中的骨干分配。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Integral membrane proteins play essential roles in a wide array ofbiological processes including nerve action potentials, homeostasis, cellarchitecture, and signal transduction. They comprise over 50% of currentand potential drug targets, yet their structural characterization haslagged far behind that of soluble proteins. For solution nuclear magneticresonance (NMR) spectroscopy, membrane proteins represent a significantchallenge because their solubilization in aqueous detergent micellesresults in particles of large size and attendant slow molecularreorientation. This limits the complexity of spectroscopy that can beemployed. We have developed a novel procedure for solubilizing membrane proteins in a surfactant system suitable for dissolution in low viscosity solvents such as pentane, butane, or propane, and are in the process of collecting 3D data for backbone assignments.