GAS MIGRATION PATHWAYS IN HYDROGENASE AND OTHER PROTEINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 许多蛋白质通过与氧气和一氧化碳等气体分子相互作用来发挥其功能。与大多数其他配体不同的是，这些气体的蛋白质反应部位往往深埋在蛋白质内部，没有明显的进入途径。描述气体路径的位置和气体迁移的机制是了解珠蛋白、加氧酶、氧化物酶等蛋白质如何工作的关键一步。然后，这些知识可以直接应用于解决具体和相关的问题。一个例子是使用氢酶商业生产用作能源燃料的氢气：了解氢酶内部的气体迁移路径为通过定向突变重新设计酶提供了关键指导，然后可以用来以负担得起的价格生产氢气。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many proteins perform their function by interacting with gas molecules such as oxygen and carbon monoxide. Unlike in the case of most other ligands, the protein reactive sites for these gases are often buried deep inside the proteins, with no obvious entry pathway. Describing the locations of the gas pathways and the mechanism of gas migration is an essential step towards understanding how proteins such as globins, oxygenases, oxidases work. The knowledge can then be directly applied to solve concrete and relevant problems. One example is the commercial production of hydrogen gas for use as an energy fuel, using hydrogenase: knowing the gas migration pathways inside hydrogenase provides crucial guidance to the re-design of the enzyme through targeted mutations, which can then be used to affordably produce H2.