DIFFUSION OF FLUORESCENTLY LABELED ENZYMES IN POLYMER FILMS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 将罗丹明标记的乙醇脱氢酶结合到Eastman聚合物薄膜中，并用FCS进行了研究。目的是确定蛋白质分子是否能够在聚合物基质中进行平移扩散。FCS数据表明，在实验条件下没有明显的扩散，也发生了亚量子光漂白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rhodamine-labeled alcohol dehydrogenase was incorporated into an Eastman polymer film and studied by FCS. The goal was to ascertain if the protein molecules were able to undergo translational diffusion in the polymer matrix. The FCS data indicated no significant diffuion and also that subtantial photobleaching occured under the experimental conditions.