GELKEYS: A SOFTWARE APPLICATION FOR 2D GEL IMAGE STORAGE, MARKUP AND SHARING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们已经创建了一个基于网络的多用户工具，用于存储2D凝胶和相关的MS数据。对于每个上传的图像，可以通过点击凝胶的表示来标记斑点；对于每个标记的斑点，可以存储多个文件。保存的图像和文件可以配置为仅限于特定的组或用户访问，允许协作，同时不公开发布数据。由于该应用程序是基于网络的，团体可以在任何他们可以访问互联网的地方共享数据。图像可以以各种格式(PNG、GIF、TIF等)上传，也可以下载带标记或不带标记的图像。最好将GelKeys作为打印和标记凝胶图像的替代方案，同时保存和组织用于演示的数据。该软件是使用ActiveState Komodo 3.5用Perl开发的。使用GD和ImageMagick Perl库来完成图像处理。所有图像和数据都存储在MySQL 5.0数据库中，并呈现在Apache2.0 Web服务器上。测试图像和MS数据文件从BUSM正在进行的项目中获得。目前，我们已经向数据库上传了18幅图像，标记了49个斑点，并存储了28个MS数据文件。增加了对图像混合等基本分析的支持，软件正在更新，以包括更多功能，包括自动斑点定量和MS数据处理。该软件提供了一个易于使用的界面，用于存储和标记2D凝胶图像上的斑点。因为它是基于Web的，所以可以从任何可以访问互联网的地方访问它。对多用户的支持和强大的安全模型允许在个人和组之间共享数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have created a multi-user web based tool for storing 2D gels and associated MS data. For every uploaded image, spots can be labeled by clicking on a representation of the gel; for each labeled spot, multiple files can be stored. Saved images and files can be configured to restrict access to only certain groups or users, allowing for collaboration while not publicly releasing data. As the application is web-based, groups can share data wherever they can access the Internet. Images can be uploaded in a variety of formats (PNG, GIF, TIF, etc), and can also be downloaded with markup and without. GelKeys can best be considered as an alternative to printing out and marking up images of gels, while simultaneously saving and organizing data for presentation. The software was developed in Perl using ActiveState Komodo 3.5. Image manipulation is accomplished using the GD and ImageMagick Perl libraries. All images and data are stored in a MySQL 5.0 database, and presented on an Apache 2.0 web server. Test images and MS data files were obtained from ongoing projects at BUSM. Currently we have uploaded 18 images to the database, labeled 49 spots, and stored 28 MS data files. Support for basic analysis such as image blending has been added, and the software is being updated to include more features including automatic spot quantitation and MS data processing. The software provides an easy-to-use interface for storing and labeling spots on 2D gel images. As it is web-based, it can be accessed from wherever one has access to the Internet. Support for multiple users and a robust security model allow for sharing of data between individuals and groups.