Facility Core C. Analytical Instrumentation

总体目标和具体目标 分析仪器设施核心的目的是支持中心研究人员进行检测、 化学品和生物分子的表征和定量。 核心设施 2 的具体目标是： 1. 提供灵敏的蛋白质鉴定和修饰分析； 2. 提供代谢物、营养素、异生物质和物质的鉴定和定量服务 各种类型的大分子损伤； 3. 实施新颖的分析方法并提高现有分析方法的灵敏度和通量 分析； 4. 提供分析方法的选择和实施咨询以及培训 使用和应用分析仪器的机会； 5. 协调开发与转化研究相关的组织和生物流体分析方法 与综合健康科学设施核心； 6. 促进伙伴关系，以改善获得高端分析仪器和技术的机会 支持中心研究的专业知识。 分析设施核心将提供复杂的质谱资源和专业知识来支持 中心研究员的研究工作。它不会不必要地重复分析能力 研究人员的实验室也不会成为大量常规分析的处理中心。的目标 核心在过去的资助期间略有变化，以反映以下领域的发展 蛋白质组学、代谢组学和生物标志物鉴定，以及对这些新技术日益增长的兴趣 由中心成员。购买主要的新仪器使我们的技术取得了显着的进步 现有服务以及扩展到应用于蛋白质、药物和代谢物分析的新型检测方法。的 该设施与分子生物学设施核心密切合作，整合所需的服务 用于蛋白质表达谱项目和血清样品分析。我们预计会有类似程度的 与拟议的综合健康科学设施核心合作，该设施将利用为 分析组织和生物流体样本以研究环境因素在人类疾病中的作用。

Overall Objectives and Specific Aims The purpose of the Analytical Instrumentation Facility Core is to support Center investigators in the detection, characterization, and quantification of chemicals and biomolecules. The specific aims of Facility Core 2 are: 1. To provide sensitive protein identification and modification analyses; 2. To offer services for the identification and quantification of metabolites, nutrients, xenobiotics, and various types of macromolecular damage; 3. To implement novel analytical methods and improve the sensitivity and throughput of existing analyses; 4. To provide consultation on the selection and implementation of analytical methods and training opportunities in usage and applications of analytical instrumentation; 5. To develop tissue and biofluid analytical assays relevant to translational research in coordination with the Integrated Health Sciences Facility Core; 6. To foster partnerships for improved access to high end analytical instrumentation and technical expertise in support of Center research. The Analytical Facility Core will provide sophisticated mass spectrometry resources and expertise to support Center Investigator research efforts. It will neither unnecessarily duplicate analytical capabilities of investigators' laboratories nor become a processing center for large numbers of routine analyses. The goals of the Core have changed slightly during the past funding period to reflect developments in the fields of proteomics, metabolomics and biomarker identification, and the increased interest in these new technologies by Center Members. Acquisition of major new instrumentation has enabled significant improvements in existing services as well as expansion into novel assays applied to protein, drug and metabolite analyses. The facility has worked in close cooperation with the Molecular Biology Facility Core to integrate services required for protein expression profiling projects and analysis of serum samples. We anticipate a similar degree of cooperation with the proposed Integrative Health Science Facility Core which will utilize assays developed for analysis of tissue and biofluid samples to investigate the role of environmental factors in human disease.