CORE--PROTEOMICS RESOURCE

该核心将为这个拟议的多囊肾病(PKD)研究小组的研究人员提供蛋白质组学技术和支持。虽然特定的基因和基因编码的蛋白质突变已被确定为ARPKD和ADPKD的起始点，但这些突变影响多个组织，因此几乎可以肯定，除了突变基因编码的主要蛋白质外，还有多种蛋白质。蛋白质组学技术可以分析初级遗传缺陷下游的蛋白质变化模式；我们将利用现有的DAB综合癌症中心(CCC)蛋白质组学/质谱学共享设施的资源和人员来提供蛋白质组学和质谱学 用于PKD研究工作的能力。这些工作将由现有的共享设施主任海伦·金博士和斯蒂芬·巴恩斯博士指导，他们在为UAB生物医学研究社区提供此类服务方面具有丰富的经验。这一核心的基本原理是，对PKD实验中以假设为导向的生物样本进行系统的蛋白质组学分析，将增强识别与PKD表型发病有关的以前未识别的蛋白质或蛋白质修饰的可能性。这样的结果可以识别用于后续研究的蛋白质，或者可以提高抗体以供PKD社区使用。这个核心的一个重要功能是为 在这方面，秘书处将向PKD社区介绍蛋白质组学技术，并使研究人员能够在知情的情况下利用核心技术，特别是对于那些以前从未接触过这些技术的人。这些活动将采取辅导和/或讲习班的形式，以及由核心主任和个别研究人员参加的咨询会。PKD这项核心服务将通过有一名生物统计学家与Core Meleth博士密切合作来加强；PKD调查人员将被强烈鼓励在进行实验之前咨询Meleth博士，以确保各种质量控制和实验设计问题得到解决。最后，将有一名研究助理专门负责这一核心，他将通过适当的2D分离和MS蛋白质鉴定来处理PKD样本。她将协调每组样本的最终数据报告的生成。这一核心还将保持 PKD蛋白质组学数据数据库，作为共享基金正在进行的努力的一部分。

This Core will provide proteomics technology and support to the investigators in this proposed polycystic kidney disease (PKD) research group. While specific genes and mutations in the proteins encoded by the genes have been identified as starting points for ARPKD and ADPKD, the mutations affect multiple tissues, and therefore almost certainly multiple proteins other than the primary ones encoded by the mutated gene. Proteomics technology allows analysis of patterns of protein changes downstream of a primary genetic defect; we will utilize the resources and personnel of the existing DAB Comprehensive Cancer Center (CCC) Proteomics/Mass Spectrometry Shared Facility to provide proteomics and mass spectrometry capabilities toward the PKD research effort. These efforts will be directed by the existing Shared Facility directors, Drs. Helen Kim and Stephen Barnes, who bring substantial experience in providing such services to the UAB biomedical research community. The rationale for this Core is that systematic proteomic analysis of biological samples from PKD experiments that are hypothesis-driven will enhance the likelihood of identifying previously unidentified proteins or protein modifications that involved in the pathogenesis of the PKD phenotype. Such results can then identify proteins for followup studies, or to which antibodies can be raised for use by the PKD community. An important function of this Core is to provide educational support to the PKD community regarding proteomics technologies and to enable informed utilization of the core technologies by the investigators, particularly for those who have not previously accessed the technologies. These will be in the form of tutorials and/or a workshop, as well as consultation sessions involving the Core directors and individual researchers. PKD this Core services will be enhanced by having a biostatistician working closely with the Core, Dr. Meleth; PKD investigators will be strongly encouraged to consult with Dr.Meleth prior to carrying out an experiment, to insure that various quality control and experimental design issues are addressed. Finally, a research associate will be dedicated to this Core, who will process PKD samples through the appropriate 2D separations and the MS protein identifications. She will coordinate the generation of reports of the final data for each set of samples. This Core will also maintain a database of PKD proteomics data, as part of an ongoing effort by the Shared Facility.