EVALUATION OF NORMALS/ BRONCHOPULMONARY LUNG LAVAGE/ BRONCHIAL BRUSHING ETC

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是从正常人的血液和肺中获得生物材料，以建立一组各种参数的正常范围。这些将提供重要的信息时，适用于个人与各种肺部疾病，并将有助于了解各种肺部疾病的病因和发病机制。 从正常个体的纤维支气管镜检查中获得的样品将如下使用：（a）从支气管肺泡灌洗（BAL）中获得的巨噬细胞将用于评价使用不同腺病毒载体的基因转移效率;（B）将进行研究以评价减少不同HIV株对巨噬细胞感染的策略;（c）巨噬细胞将根据腺病毒载体感染后细胞因子释放的模式进行评价;（d）将用通过刷肺气道收集的气道上皮细胞进行与（a）和（c）中所述的实验类似的实验;（e）评估通过BAL收集的肺上皮衬里液的抗腺病毒抗体、各种细胞因子和血管内皮生长因子水平; (f)将使用微阵列对收集的气道细胞进行各种基因表达研究，以评估吸烟对肺气道内衬细胞（气道上皮细胞）中抗氧化剂和其它基因表达的影响，以及这些变化如何与肺癌相关基因突变变化的频率相关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to obtain biologic materials from the blood and lungs of normal individuals to establish a set of normal range for various parameters. These will provide important information when applied to individuals with various pulmonary diseases, and will help in understanding of the etiology and pathogenesis of various lung diseases. Samples obtained from fiberoptic bronchoscopy in normal individuals will be used as follows: (a) macrophages obtained from bronchoalveolar lavage (BAL) will be used to evaluate efficiency of gene transfer using different adenovirus vectors; (b) studies will be conducted to evaluate strategies to decrease infection of macrophages by different strains of HIV; (c) macrophages will be evaluated in terms of pattern of cytokine release following infection with adenovirus vectors; (d) similar experiments as the ones described in (a) and (c) will be conducted with airway epithelial cells collected by brushing the lung airways; (e) lung epithelial lining fluid collected by BAL will be evaluated for anti-adenovirus antibodies, for various cytokines and for vascular endothelial growth factor levels; (f) collected airway cells will be evaluated for the various gene expression studies using microarrays for the effects of smoking in the expression of antioxidant and other genes in the cells lining the airways of the lungs (airway epithelial cells) and how these changes correlate with the frequency in mutation changes of genes that are relevant to lung cancer.