ASSOCIATION BETWEEN INTESTINAL MICROFLORA AND MUCOSAL INFLAMMATION IN IBS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这是一项初步研究，旨在制定收集和分析肠易激综合征患者和健康对照者肠道菌群和亚临床炎症的方案。这项研究正在寻找收集生物标本，粪便，结肠粘膜样本和血液。这项研究的方案和初步数据将用于制定未来的方案和提交更大的赠款，我们的目标是调查肠道细菌（微生物菌群）的正常平衡是否起着重要作用。 如果肠道微生物菌群的变化导致粘膜炎症和粘膜免疫系统的激活，以及如果这些变化导致GI功能和功能性症状的改变，则可能在IBS中起作用。此外，这项研究将有助于确定IBS中的亚临床肠道炎症是否可以通过测量外周血和粪便样本中的炎症标志物（即，如果粪便和外周血中更实用的炎症生物标志物与通过粘膜细胞因子测量炎症的金标准相关）。该研究将招募10名IBS受试者和10名健康对照 科目

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a pilot study to develop protocols for collection and analysis of intestinal microflora and subclinical inflammation in patients with Irritable bowel syndrome and healthy controls. The study is looking at collecting biological specimens, stool, colonic mucosal samples, and blood. The protocols and preliminary data from this study will be used for development of future protocols and submission of larger grants in which we aim to investigate if a normal balance of intestinal bacteria (microflora) plays a role in IBS, if changes in intestinal microflora lead to mucosal inflammation and activation of the mucosal immune system, and if these changes lead to alterations in GI functions and functional symptoms. In addition, this study will help determine if sub-clinical intestinal inflammation in IBS can be assessed by measuring inflammatory markers in peripheral blood and stool samples (i.e., if the more practical biomarkers for inflammation in stool and peripheral blood correlate with the gold standard measure of inflammation by mucosal cytokines). The study will recruit 10 IBS subjects and 10 healthy control subjects.