MYOCARDIAL INTERCALATED DISKS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这个项目将检验一个假设，即一个蛋白质复合体在心肌间盘处组装。该蛋白复合体由酪氨酸激酶ErbB2和Src、蛋白磷酸酶1/2和p21激活的蛋白激酶PAK1和PAK2组成。这个复合体调节连接蛋白43的磷酸化，连接蛋白43是一种调节相邻心肌细胞之间的离子和电子通讯的蛋白质。这一假说将通过共聚焦显微镜和免疫共沉淀来检验，以评估该复合体中的蛋白质-蛋白质相互作用。这项研究将利用一只心肌缺血的兔子和一只扩张型心肌病的仓鼠模型来检验这一假设。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project will examine the hypothesis that a protein complex is assembled at the myocardial intercalated disk. The proposed protein complex is composed of the tyrosine kinases, ErbB2 and Src; protein phosphatases 1/ 2 and the p21 activated kinases, PAK1 and PAK2. This complex regulates the phosphorylation of connexin 43, a protein that mediates the ionic and electrical communication between adjacent cardiomyocytes. This hypothesis will be examined by confocal microscopy and co-immunoprecipitation, to assess protein- protein interactions within this complex. This study will utilize a rabbit of myocardial ischemia and a hamster model of dilated cardiomyopathy to examine this hypothesis.