MECHANISMS OF TRANSCRIPTIONAL COORDINATION AMONG PHOSPHORYLASE KINASE GENES

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 磷酸化酶激酶（PhK）是参与糖原分解途径的关键调节酶，通过磷酸化催化磷酸化酶的活化。具有亚基组成（α、β、γ、δ）4的十六聚体酶用于整合激素、代谢和神经信号，导致肌肉和肝脏中糖原储存的分解。PhK的每个亚基由单独的基因编码，并且所有亚基都存在多种组织特异性同种型。虽然已经做了很多工作来阐明PhK催化的生化调节，但关于这种复杂寡聚体的转录调节的信息很少。我们的工作旨在阐明负责的时间和空间调控的PhK基因的表达，通过提供所有人类α，β和γ启动子的识别和功能分析的元素。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Phosphorylase kinase (PhK) is a key regulatory enzyme involved in the glycogenolytic pathway, catalyzing the activation, by phosphorylation, of phosphorylase. The hexadecameric enzyme, with subunit composition (alpha,beta,gamma,delta)4, serves to integrate hormonal, metabolic and neural signals, leading to the breakdown of glycogen stores in muscle and liver. Each subunit of PhK is encoded by a separate gene, and multiple tissue specific isoforms exist for all of the subunits. While much work has been done to elucidate the biochemical regulation of PhK catalysis, little information regarding transcriptional regulation of this complex oligomer is available. Our work seeks to elucidate those elements responsible for the temporal and spatial regulation of PhK gene expression by providing for the identification and functional analysis of all human alpha, beta and gamma promoters.