Core--Microscopy

核心将提供电子和光学显微镜成像，这对于实现各个项目的目标是必不可少的。蛋白质在细胞内的定位，转录因子的核质运输，以及肌球蛋白细丝的成像是检验该计划假设的关键，需要广泛使用共聚焦显微镜和电子显微镜。该核心将为个别研究人员提供培训，调整和校准这些仪器，协助准备标本，并监督实验和维护的时间表。核心包括一个由当地显微镜专家组成的小组，他们将进行培训并实施最新的升级和创新。核心员工将确保计划 使这些资源得到有效和高效的使用。显微镜的一个共同核心带来了其他好处，如共享方案和试剂，交换正在进行的实验的信息，以及在不同项目的工作人员之间培养友谊，因为他们使用共同的设施和方法来研究相关问题。

The Core will provide electron and light microscopy imaging that is essential to accomplishing the goals of the individual projects. The intraceltular localization of proteins, nucleo-cytoplasmic transport of transcription factors, and imaging of myosin filaments are key to testing the hypotheses of this Program, and will require extensive use of confocal microscopy and electron microscopy. The core will provide training for individual investigators, align and calibrate these instruments, assist with preparation of specimens, and oversee scheduling of experiments and maintenance. The core includes a panel of local microscopy experts, who will conduct training and implement the latest upgrades and innovations. The Core staff wilt ensure that the Program gets effective and efficient use of these resources. A common core for microscopy brings with it other benefits in terms of sharing of protocols and reagents, trading of information on experiments in progress and nurturing a sense of camaraderie between individuals working on different projects, as they use common facilities and methodology to study related issues.