CORE: SIGNAL TRANSDUCTION CORE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 受体-配体的相互作用和信号转导生物化学的诱导是细胞生物学中所有调控事件的驱动力。这种调节在免疫系统中尤其明显，免疫系统表现出大量的受体-配体相互作用，促进免疫细胞的分化、增殖、凋亡和效应器功能的激活。因此，信号转导生物化学是理解免疫细胞稳态内生物事件的核心。 在过去的十年里，免疫细胞信号传递的知识体系已经相当成熟，特别是随着酪氨酸蛋白激酶在调节蛋白质-蛋白质相互作用中的中心作用的发现。由于这是一个新领域，用于研究由细胞因子或其他免疫细胞激活剂诱导的信号事件的技术相当专业，可能不容易被缺乏经验的实验室在没有直接支持的情况下获得。考虑到从一种受体到另一种受体的分析方法的相似性，我们预计那些研究相关信号系统的人可以从过去的经验中受益。因此，该应用程序建议继续支持核心设施，以容纳专注于免疫细胞信号转导生物化学的专业知识和设备。它将被用作知识库和技术资源，供发现需要探索各种免疫反应中的信号事件的新研究人员使用，并将促进专业知识和特定分析的开发和向研究其他信号网络的研究人员转移。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Receptor-ligand interactions and the induction of signal transduction biochemistry are the driving force for all regulatory events in cell biology. This regulation is especially evident in the immune system, which exhibits numerous receptor-ligand interactions that promote immune cell differentiation, proliferation, apoptosis, and activation of effector functions. Accordingly, signal transduction biochemistry is central to understanding the biological events within immune cell homeostasis. The body of knowledge of immune cell signaling has matured considerably over the past ten years, especially with the discovery of the central role for tyrosine protein kinases in regulatory protein-protein interactions. Since it is a nascent field, the techniques used to study signaling events induced by cytokines or other immune cell activators are fairly specialized and perhaps not readily acquired by inexperienced laboratories without direct support. Given the similarity of assays that apply from one receptor to another, we expect benefit from past experience for those who study related signaling systems. Thus, this application proposes to continue to support a core facility to house expertise and equipment that focuses on immune cell signal transduction biochemistry. It will be used as a knowledge-base and technical resource for new investigators who find a need to explore signaling events in various immune responses, and will facilitate development and transfer of expertise and specific assays to researchers studying other signaling networks.