Development of Collagen-Based Engineered Blood Vessels

描述（由申请人提供）：静电纺丝是一种非常有前途的技术，用于制造组织支架，特别是用于血管的组织支架。在这里，我们提供了强有力的初步数据，证明了将胶原蛋白和其他基质材料旋转成适合细胞播种的适当几何形状的可行性和创新性。传统的移植材料容易感染和血栓形成，特别是用于小血管重建时。因此，对更好的导管材料的需求越来越大。这个项目将帮助我们把一种生物替代假肢移植物带到诊所。结合独特的支架系统、体内评估和生物反应器开发，该项目的成功完成将为血管置换带来一种全新的方法，有可能改变血管置换的模式，并解决每年近140万例动脉置换的需求。项目的叙述

DESCRIPTION (provided by applicant): Electrospinning is a technology that is very promising for fabricating tissue scaffoldings, particularly those intended for blood vessels. Here we have presented strong preliminary data demonstrating the feasibility and innovation of spinning collagen and other matrix materials into the appropriate geometry for cell seeding. Traditional graft materials are prone to infection and thrombosis particularly when used for small vessel reconstructions. Thus, there is a large and growing need for better conduit materials. This project will help us to bring a biological alternative to prosthetic grafts to the clinic. With its combination of a unique scaffold system, in vivo evaluation, and bioreactor development, successful completion of this project will result in a fundamentally new approach to vascular replacement with potential to change the paradigm for vascular replacement, and address the need for the almost 1.4 million arterial replacements performed per year. Project Narrative This project aims to generate a scaffold for vascular graft replacement using electrospun biological polymers. The scaffold will be developed and cultured with vascular cells in a bioreactor capable of controlled pulsatile flow. The scaffold will be evaluated in an in vivo rabbit abdominal aorta replacement model.