Microarray Core

微阵列核心的目的是帮助风湿病核心中心的科学家使用 高通量基因表达谱分析方法，以解决生物学中的问题， 风湿性疾病风湿病核心中心微阵列核心包括单独的 现有的Affytelum和cDNA微阵列核心中的组件，也为研究者提供服务 附属于密歇根大学综合癌症中心和所有医学院的研究人员。 该联合核心的主任是Jill A. Macoska，泌尿系副教授 密歇根大学医学院的教授，自2000年以来一直担任核心主任。核心 帮助研究者设计、进行和分析基因表达谱实验 使用基于寡核苷酸和cDNA的微阵列技术和定量聚合酶链反应 技术.核心还提供基因组学研究的工具，包括SNP单倍型分析服务， Affyscore HuSNP基因芯片服务。核心的具体目标是：1。协助研究者进行实验设计和实施。2.为微阵列和基因芯片实验提供探针制备、标记、杂交、洗涤和数据采集。3.协助研究者进行定量PCR研究和SNP基因分型实验。4.提供统计和生物信息学支持。5.为实验室人员提供培训，以执行核心中使用的实验技术。

The purpose of the Microarray Core is to assist Rheumatic Diseases Core Center scientists in the use of high-throughput gene expression profiling methods towards the solution of problems in the biology of rheumatic diseases. The Rheumatic Diseases Core Center Microarray Core comprises a separate component within the existing Affymetrix and cDNA Micorarray Core, which also serves investigators affiliated with the University of Michgan Comprehensive Cancer Center and all medical school investigators. The Director of this combined Core is Dr. Jill A. Macoska, Associate Professor in the Department of Urology in the University of Michigan School of Medicine, who has served as the Core Director since 2000. The Core exists to assist investigators with the design, conduct and analysis of gene expression profiling experiments using oligonucleotide- and cDNA-based microarray technology and quantitative polymerase chain reaction technology. The Core also offers tools for genomics studies, including a SNP haplotyping service and Affymetrix HuSNP GeneChip services. The Specific Aims of the Core are: 1. Assist investigators with experimental design and implementation. 2. Provide probe preparation, labeling, hybridization, washing, and data acquisition for microarray and GeneChip experiments. 3. Assist investigators with quantitative PCR studies and SNP genotyping experiments. 4. Provide statistical and bioinformatics support. 5. Provide training for lab personnel to perform experimental techniques used in the Core.