FLOW CYTOMETRY RESOURCE

作为一个完全由癌症中心管理的机构，流式细胞术共享资源帮助HICCC成员 基于流式细胞术的研究。服务包括： 荧光激活细胞分选(FACS) ·流式细胞术用于免疫表型分析 流式细胞术分析细胞周期和细胞凋亡 该设施的产能在过去三年中由于收购了 新乐器。该设施现在运行着五台仪器，包括两台荧光激活的细胞 允许高速细胞分选并可以检测多达12个荧光参数的分选器，以及一个 先进的细胞分析仪，除了多色荧光外，还能够执行特殊应用，如 作为DMA含量的测定，多重(活体)荧光蛋白分析和钙离子通量 测量。除了这些复杂的仪器外，该设施还提供两个用户操作的通道 基础细胞仪分别允许3色或4色免疫表型以及细胞周期 并进行细胞凋亡检测。在过去的几年里，该设施的服务使130个中央管理委员会受益 调查人员，其中HICCC成员是仪器的主要用户，约占60%。 高使用量使设施能够以较低的费用提供服务，费用将进一步降低 HICCC成员。该设施中可用的尖端设备使哥伦比亚大学的调查人员能够 在流式细胞术领域进行最先进的研究，因此是 HICCC成员。未来，我们计划升级流式细胞仪，以方便检测和分选 新开发的活的彩色荧光蛋白和购买一台仪器，作为一种灵活的 用于其他基于荧光的技术的分析平台，包括细胞学珠粒分析(CBA) 测量细胞因子的产生，以及其他潜在的应用。 在CCSG的最后一段时间里，使用该设施的调查人员中有36%是癌症中心 有同行审查资金的成员，这些成员占使用情况的50%至66% 可提供的3项服务。该设施的拟议总运营预算为425,004美元，其中我们 向CCSG索要116,271美元

A fully Cancer Center-managed facility, the Flow Cytometry Shared Resource assists HICCC members in flow cytometry-based studies. Services include: ¿ Fluorescence-activated cell sorting (FACS) ¿ Analytical flow cytometry for immunophenotyping ¿ Flow cytometry for analyzing cell cycle and apoptosis The capacity of the facility has increased substantially over the past three years due to the acquisition of new instruments. The facility now operates five instruments, including two fluorescence-activated cell sorters that allow high-speed cell sorting and can detect up to 12 fluorescent parameters, and one advanced cell analyzer that besides multi-color fluorescence is able to perform specialty applications such as determination of DMA content, multiple (living color) fluorescent protein analysis and calcium flux measurements. In addition to those sophisticated instruments, the facility provides access to two useroperated basic cytometers permitting 3 or 4 color immunophenotyping, respectively, as well as cell cycle and apoptosis assays. Over the last several years, the services of the facility have benefited 130 CUMC investigators, with HICCC members comprising the main users of the instruments accounting for ~60%. The high usage volume allows the facility to offer the services at low fees, which are further reduced for HICCC members. The sophisticated equipment available in the facility allows investigators at Columbia to perform state-of-the-art research in the area of flow cytometry, and therefore represent a vital resource for HICCC members. In the future, we plan to upgrade the flow cytometers to facilitate detection and sorting of newly developed living color fluorescent proteins and to purchase an instrument that serves as a flexible analysis platform for additional fluorescence-based techniques, including cytometric bead assays (CBA) to measure cytokine production, among other potential applications. During the last period of the CCSG, 36% of the investigators using the facility were Cancer Center members with peer-reviewed funding, with those members representing from 50% to 66% of the usage of the 3 available services. The proposed total operating budget of the facility is $425,004, of which we are requesting $ 116,271 from the CCSG