Morphology and Molecular

形态学和分子生物学核心将提供该计划中的各种项目， 进行常规形态学和分子生物学检查所需的专业知识、设施和支持 程序。技术人员、研究生和博士后研究员的培训是 核心C。核心C的形态学部分提供的服务包括常规光学显微镜检查， 免疫荧光显微术和电子显微术。核心工作人员将协助收购和 形态学数据的解释。其他专门的形态学和免疫组织化学支持将 根据需要制定。核心C的这个组件将支持该计划中的所有项目。的服务 核心C的分子生物学部分提供的技术将包括DNA的生长和纯化， 重组病毒; cDNA的定点诱变，转染载体和质粒的产生， 哺乳动物细胞培养物中通道和连接蛋白的表达、分析以及初级 新生儿心肌细胞的培养物。当需要时，将设计和测试RNA沉默构建体 在使用前，核心。核心也将是生产和维护的主要设施， 转基因小鼠群体。这些职责将包括育种，基因分型和表型分析 免疫化学方法。核心C的这一构成部分将主要支持项目2和3。

The Morphology and Molecular Biology Core will provide the various projects in the program with the necessary expertise, facilities, and support to perform routine morphological and molecular biological proceedures. Training of technicians, graduate students and post-doctoral fellows is an integral aspect of Core C. The services provided by the Morphology component of Core C include routine light microscopy, immunofluorescent microscopy and electron microscopy. Core staff will assist in both the acquisition and interpretation of morphological data. Other specialized morphological and immunohistochemical support will be developed as required. This component of Core C will support all projects in the program. The services provided by the Molecular Biology component of Core C will include the growth and purification of DNA and recombinant viruses; site directed mutagenesis of cDNA, production of transfection vectors and plasmids, expression, analysis of channel and junction proteins in mammalian cell cultures and production of primary cultures of neonatal cardiac myocytes. When needed, RNA silencing constructs will be designed and tested by the Core prior to use. The Core will also be the primary facility for the production and maintenance of the transgenic mouse colonies. These responsibilities will include breeding, genotyping and phenotypic analysis by immunochemical means. This component of Core C will primarily support projects 2 and 3.