Research Histopathology

自1981年以来，一个集中的组织学实验室为MDACC的研究人员提供组织学服务。 研究组织病理学机构(RHF)的目的是提供技术支持和咨询， 开发和应用适当的新技术以支持不断发展的研究需求，并保持 实施这些基于组织的技术需要一致性和高质量。RHF进程已修复 常规冷冻标本包括细胞系和小鼠胚胎标本进行组织学分析， 免疫组织化学和原位杂交薄厚切片；特殊染色；组织转移； 无DNA酶和无核糖核酸酶程序；冰冻切片；组织微阵列切片；手术刀 显微解剖和免疫组织化学染色。MDACC的所有教职员工都可以参加RHF， 优先考虑获得同行评议拨款的调查人员。RHF最近占地740平方英尺 北校区病理学系翻新后的实验室空间和R.E.的370平方英尺。 南校区的“鲍勃”史密斯研究大楼。在提供的服务中，包括快速准备 高质量的标准组织切片；根据所选项目的需要应用特殊技术， 包括制备冰冻切片和组织切片，用于组织化学、免疫组织化学和免疫组织化学 原位杂交分析；咨询研究人员以适应他们的技术和方法 特殊需要；转介给器官现场的副专科病理学家进行咨询 组织病理学。激光捕获显微解剖服务将于2007年秋季增加。RHF利用 最先进的设备来完成其工作，因为在过去两年中，两个实验室都更换了 他们的设备，以更好地服务于他们的用户。RHF目前维护的工作人员包括一名共享的 资源主任、联席主任、经理/首席技术员、2名高级组织技术员、2名 研究组织学技术员和2名组织学实验室助理。在过去的5年里，RHF 由代表18个不同CCSG项目的287名调查人员使用，使用量增加了569%。 获得同行评审资金的用户占服务利用率的78%，占用户的53%。 今后的计划包括继续改进组织学计费数据库系统，继续 设备更换周期、扩建南校区设施和启动激光捕获 显微解剖服务。

Since 1981, a centralized histology laboratory has provided histologic services to investigators at MDACC. The aim of the Research Histopathology Facility (RHF) is to provide technical support and consultation, develop and apply appropriate new techniques to support evolving research needs, and maintain the consistency and high quality needed to perform these tissue-based techniques. The RHF processes fixed and frozen specimens including cell line and mouse embryo preparations for histologic analysis in routine, thin and thick sections for immunohistochemistry and in situ hybridization; special staining; tissue transfer; DNAse- and RNAse-free procedures; frozen sections; sectioning of tissue microarray blocks; scalpel microdissection; and immunohistochemical staining. The RHF is available to all faculty members at MDACC, with preference given to investigators with peer-reviewed grants. The RHF occupies 740 sq ft of recently renovated laboratory space in the Department of Pathology on the North Campus and 370 sq ft in the R.E. "Bob" Smith Research Building on the South Campus. Among the services provided are rapid preparation of high-quality standard histologic sections; application of special technologies as needed for selected projects, including preparation of frozen sections and tissue sections for histochemical, immunohistochemical, and in situ hybridization analysis; consultation with investigators to tailor technologies and methodologies to their special needs; and referral for consultation by organ-site subspecialty pathologists on interpretation of histopathology. Laser capture microdissection services will be added in the Fall of 2007. The RHF utilizes state-of-the-art equipment to complete its work, as during the past 2 years both laboratories have replaced their equipment to better serve their users. The RHF currently maintains a staff that includes a Shared Resource Director, a Co-Director, a Manager/Chief Technologist, 2 Senior Histology Technicians, 2 Research Histology Technicians, and 2 Histology Laboratory Assistants. During the past 5 years, the RHF was used by 287 investigators representing 18 different CCSG Programs, and use increased by 569%. Users with peer-reviewed funding accounted for 78% of service utilization and represented 53% of users. Future plans include continued improvement of the Histology Chargeback Database System, continuation of the equipment replacement cycle, expansion of the South Campus facility, and activation of laser capture microdissection services.