Shared Resources-Animal Genomics

动物基因组学共享资源(AGS)的目的是创建(并且通过冷冻保存， 维护)用于包括癌症在内的人类疾病的转基因和基因敲除模型。具体目标是： 1)提供转基因和靶向构建设计方面的咨询；2)将DNA导入受精卵或 胚胎干细胞；3)创造包含所需DNA添加或 改变；以及4)冷冻保存小鼠胚胎，用于成本效益高的长期保存基因工程 老鼠。转基因小鼠服务创建于1989年。添加了创建基因敲除小鼠的能力 1997年。自2000年以来，已经进行了小鼠胚胎的冷冻保存。创造新奇的鼠标 通过转基因或同源重组的菌株，允许耶鲁大学癌症的许多成员 中心(YCC)，以确定基因的正常功能以及该功能的扰动如何导致 异常发育或疾病，如癌症。调查人员可以使用AGS创建直接类似于 突变与人类疾病有关，从而提供了可以研究人类疾病的模型 受试者不能。创造这种诱导突变的能力使研究人员能够有效地利用小鼠 遗传学解剖和分析复杂的发育途径、生化过程和 哺乳动物体内的生物分子相互作用达到了在这种技术存在之前无法达到的水平。 这使研究人员能够在比体外方法更深的水平上研究生物过程，如 在生物化学或细胞培养允许的情况下，通过出现新的表型 诱导突变，发现这些过程和途径之间的新关系。超低温保存 老鼠品系使研究人员能够更有效地利用他们的财务资源进行研究，同时 保存有价值的菌株以备将来使用。YCC AGS每年生产40-60个转基因小鼠品系， 并每年创建15-20个新的靶向突变小鼠模型。 自以来，动物基因组服务一直是YCC的共享资源。AGS进入新的空间 随着2002年Anylan中心大楼的竣工，专门为它们设计的。 2005年，28名YCC调查人员使用了AGS，总共大约。资源总使用率的62%。全 2005年YCC的使用来自8个YCC研究项目中的7个经过同行评审的科学家

The purpose of the Animal Genomics Shared Resource (AGS) is to create (and through cryopreservation, maintain) transgenic and knockout models for human diseases including cancer. The specific objectives are: 1) Provide consultation on transgene and targeting construct design; 2) Introduce DNA into zygotes or embryonic stem cells; 3) Create founder animals or chimeras that incorporate the desired, DNA addition or change; and 4) Cryopreserve mouse embryos for cost-effective, long-term storage of genetically engineered mice. The Transgenic mouse service was created in 1989. The ability to create knockout mice was added in 1997. Cryopreservation of mouse embryos has been performed since 2000. The creation of novel mouse strains, either by transgenesis or homologous recombination, allows many members of the Yale Cancer Center (YCC), to determine the normal function of genes and how perturbation of that function can result in aberrant development or diseases such as cancer. Investigators can use AGS to create direct analogs of mutations involved in human disease, thus providing models that can be studied in a way that human subjects cannot. The ability to create such induced mutants allows investigators to effectively use mouse genetics to dissect and analyze complicated developmental pathways, biochemical processes, and biomolecular interactions in a mammal to a level previously unattainable before such technology existed. This enables researchers to investigate biological processes at a far deeper level than in vitro methods such as biochemistry or cell culture will allow, and through the appearance of novel phenotypes resulting from induced mutations, discover new relationships between these processes and pathways. Cryopreservation of mouse strains allows investigators to more efficiently utilize their financial resources in research while maintaining valuable strains for future use. The YCC AGS produces 40-60 transgenic mouse strains/year, and creates 15-20 new targeted mutant mouse models/year. Animal Genomics Services has been a shared resource of the YCC since 1989. AGS moved into new space designed specifically for their use with the completion of The Anylan Center building in 2002. Twenty-eight YCC investigators used AGS in 2005 for a total of approx. 62% of all usage of the resource. All YCC usage during 2005 was from peer-reviewed scientists from 7 of 8 YCC Research Programs