Intravital Microscopy

细胞黏附和迁移机制已被认为是调节命运的关键因素 和所有白细胞的功能。因此，细胞黏附属性的直接可视化和精细 影响这些特性的结构特征是我们理解白细胞如何 参与炎症、止血、T血栓形成和免疫。活着的显微镜核心已经被 ：根据PPG调查人员的明确需求进行模拟，旨在利用相当大的 其关键人员经验丰富，能提供专业技术和专业设备的设计和实施 糖基转移酶的病理生理作用的体内成像研究。 活体显微镜核心的具体目标如下： 1.进行和分析基于活体显微镜的小鼠组织黏附和迁移研究。 目前可用的影像模型包括：提睾肌；耳皮肤；骨髓；肝脏； 腹股沟淋巴结；膀胱；膝关节；小肠，包括肠系膜、Peyer‘s补片 和固有层。 2.提供通道和专业知识，并利用多光子进行具体实验 基于显微镜的血管内外血细胞黏附、迁移和细胞间记录 多达六个维度(即空间、时间、颜色、荧光强度)的相互作用。 4.为体内血细胞归巢的计划、实施和分析提供协助 明确的炎症模型。 成像核心将提供的服务将允许参与的调查人员调查如何定义 糖基化途径影响血细胞与其环境的相互作用的分辨率范围为 从单个细胞到完整的动物。

Cellular adhesion and migration mechanisms have been recognized as crucial elements regulating the fate and function of all leukocytes. As such, the direct visalization of cell adhesion properties and the fine structural feature that influence these properties is central to our understanding of how white blood cells Darticipate in inflammation, hemostasisT thrombosis and immunity. The Intravital Microscopy Core has been :ormulated based on a stated need of PPG investigators and is designed to draw on the considerable experience of its key personnel to provide expertise and specialized equipment in the design and execution of in vivo imaging studies on the pathophysiological role of glycosyltransferases. The Specific Aims of the Intravital Microscopy Core are as follows: 1. To execute and analyze intravital microscopy-based adhesion and migration studies in murine tissues. Currently available imaging models include: cremaster muscle; ear skin; bone marrow; liver; popliteal and inguinal lymph node; bladder; knee joint; and small intestine, including mesentery, Peyer's patches and lamina propria. 2. To provide access to and expertise and to conduct specific experiments utilizing multi-photon microscopy-based recording of intra- and extravascular blood cell adhesion, migration and cell-cell interactions in as many as six dimensions (i.e. space, time, color, fluorescence intensity). 4. To provide assistance in the planning, execution and analysis of blood cell homing in vivo employing defined models of inflammation. Services to be provided by the Imaging Core will allow participating investigators to investigate how defined glycosylation pathways influence blood cells interactions with their environment at a resolution ranging from single cells to intact animals.