Imaging & microscopy

成像和显微镜核心的主要目的是通过提供 获得成像和显微镜设备，这可能是昂贵的采购和维护， 个别研究人员的实验室不专门用于这些技术。这使调查人员能够扩大 在神经科学研究所内进行研究的技术， 费用将由研究者个人、神经科学研究所和莫尔豪斯学校分担 医学。 成像核心可提供明场、荧光和共聚焦显微镜和成像设备 采集硬件和软件的设计允许相关图像的生成和分析 原位杂交、免疫细胞化学、组织学以及凝胶数字化和分析 通过分子技术如Northern、Western、Southern、PCR和RT-PCR产生。图像 出版物的质量。 在U 54参与者中，戴维森实验室将使用低温恒温器和显微镜成像 对从轮班小鼠中采集的组织样本进行病理学分析的核心设备 光的时间表。保罗实验室将使用成像核心中的低温恒温器对脑组织进行原位切片 放射自显影图像的杂交和随后的光密度分析。此外，保罗 实验室将使用共聚焦显微镜，以协助映射表达的GABAA亚基表达， 使用新的GABAA亚单位抗体的视交叉上核和其它下丘脑核。的 福原爱实验室将使用明场和荧光显微镜检查是否Bmall-荧光素酶 利用原位杂交和图像分析软件成功地将DNA递送到成纤维细胞 由核心提供。在其他神经科学专家的适当指导下，也可以使用核心设备 研究所研究人员和其他莫尔豪斯医学院人员。

The major aim of the Imaging and Microscopy Core is to facilitate the research of investigators by providing access to imaging and microscopy equipment that might be expensive to procure and maintain in an individual investigator's laboratory not dedicated to these techniques. This allows investigators to broaden the techniques available for their research within the Neuroscience Institute by allowing the burden of expenses to be shared between individual investigators, the Neuroscience Institute and Morehouse School of Medicine. The imaging core can provide equipment for brightfield, fluorescence and confocal microscopy and image acquisition. The hardware and software are designed to allow production and analysis of images associated with in situ hybridization, immunocytochemistry, histology, as well as digitization and analysis of gels produced from molecular techniques such as Northerns, Westerns, Southerns, PCR and RT-PCR. Images produced are publication quality. Among the U54 participants, the Davidson laboratory will use the cryostat and microscope imaging equipment of the core to perform pathological analyses on tissue samples harvested from mice on shifting light schedules. The Paul laboratory will use the cryostat in the imaging core to section brain tissue for in situ hybridization and subsequent optical density analysis of autoradiograph images. In addition, the Paul laboratory will use confocal microscopy to assist in mapping expression of the GABAA subunit expression in the suprachiasmatic nucleus and other hypothalamic nuclei using novel GABAA subunit antibodies. The Fukuhara laboratory will use brightfield and fluorescence microscopy to examine whether Bmall-luciferase DNA is successfully delivered to fibroblasts utilizing in situ hybridization and image analysis software provided by the core. Core equipment can also be used, under proper guidance by other Neuroscience Institute investigators and other Morehouse School of Medicine personnel.