Post-transcriptional Regulation of NO Synthase

一个重要的血管适应层流剪切应力是内皮细胞一氧化氮合酶（eNOS）的表达增加。这不仅发生在培养的内皮细胞中，也发生在暴露于高水平血流的血管中，比如在运动训练期间。我们实验室的研究表明，eNOS对剪切应力的上调通过两种不同的途径发生，这两种途径都依赖于酪氨酸激酶cSrc。一种途径导致依赖于经典ERK1/2途径的转录短暂增加一小时。在此之后，持续的剪切应力导致eNOS mRNA的长时间稳定，而不依赖于ERK1/2或其上游信号。调控eNOS mRNA稳定性的机制仍不明确。在初步研究中，我们发现层状剪切应力显著增加eNOS转录本的3'聚腺苷化；以挪士3 '

An important vascular adaptation to laminar shear stress is increased expression of the endothelial cell nitric oxide synthase (eNOS). This not only occurs in cultured endothelial cells, but it also occurs in vessels exposed to high levels of flow, such as during exercise training. Research from our laboratory has shown that eNOS upregulation in response to shear stress occurs via two divergent pathways, both of which are dependent on the tyrosine kinase cSrc. One pathway leads to a brief one hour increase in transcription that is dependent on a classical ERK1/2 pathway. Following this, continued shear stress leads to a prolonged stabilization of the eNOS mRNA that is independent of ERK1/2 or its upstream signals. The mechanisms regulating eNOS mRNA stability remain poorly defined. In preliminary studies, we have found that laminar shear stress dramatically increases the 3' polyadenylation of eNOS transcripts; eNOS 3' poly(A) tails of 75 nt to 160 nt are expressed in response to shear stress compared to poly(A) tails of <25 nt under basal conditions. ENOS transcripts with longer poly(A) tails are more stable and are translationally more active than those with short poly(A) tails. This modulation of RNA 3' processing seems to represent a completely novel mechanism for regulation of gene expression and translation in response to mechanical stimuli. In the proposed studies, we plan to gain additional insight into this phenomenon. In aim one, we will identify the hexanucleotide element in the eNOS polyadenylation signal and determine its role in shear-induced polyadenylation. We will examine the polyadenylation and cleavage efficiency of eNOS constructs containing wild-type sequence element and compare it to eNOS constructs with canonical sequence element. This will be studied using in vitro assays and in eNOS-/- cells exposed to shear. In aim 2, we will examine conserved sequences upstream to the eNOS poly(A) signal for their responsiveness to shear by making sequential deletions of this region and examining polyadenylation efficiency in vitro and in cells. We have preliminary data suggesting that HMG CoA reductase inhibitors also increase eNOS 3' poly(A) tail length. In aim 3, we will examine mechanisms responsible for this phenomenon. In this aim, we will determine the roles of the small G protein Rho, its target Rho kinase, and cytoskeleton organization in eNOS mRNA 3' poly(A) tail processing. Finally, in aim 4, we will determine if shear stress associated with exercise training modulates eNOS 3' polyadenylation in vivo. In these experiments, we will exercise train wild-type mice and mice heterozygotic for cSrc (in whom eNOS expression is not increased by exercise). Overall, these studies promise to provide very novel information regarding how mechanical forces and other stimuli modulate gene expression via regulation of mRNA 3' poly(A) tail length.