Biosecurity Enhancements for the Rocky Mountain Regional Biocontainment Laborator

描述（由申请人提供）：本提案概述了科罗拉多州立大学落基山地区生物控制实验室（RMRBL）的能力建设计划。RMRBL是科罗拉多州立大学山麓校区传染病研究中心（IDRC）的一个组成部分。到2010年，IDRC将提供近2.5万平方英尺。为满足30多名现任首席研究员和十几个合作机构的需求，提供了大约100平方米的动物空间。IDRC支持在动物生物安全2级和3级对选定药剂、新出现的病原体、虫媒病毒和分枝杆菌疾病进行研究，因此，它是落基山脉地区乃至全国的一个独特设施。我们在此建议增加RMRBL笼子清洗设施的高压灭菌器容量，以优化动物笼子和设备的生物安全和生物安全灭菌。建议在RMRBL动物设施附近安装一个大型直通式高压灭菌器，在主净化高压灭菌器失效的情况下，也可以进行紧急冗余灭菌。拟议设备的尺寸和占地面积是根据位于校园另一个笼式洗涤设施的高功能高压灭菌器建模的，允许设备冗余和拟议机器的概念验证。还要求为可移动动物笼提供资金，以便为老鼠以外的物种提供高水平的封闭住房，并为动物在饲养区和操作空间之间的移动提供运输住房。总的来说，这一建议将使IDRC装备齐全，考虑到动物使用的预期增长，以及高效和有效地处理在现场进行的专门研究所需设备的能力。

DESCRIPTION (provided by applicant): This proposal outlines a plan for capacity building at the Rocky Mountain Regional Biocontainment Laboratory (RMRBL) at Colorado State University. The RMRBL is one component of the CSU Foothills Campus Infectious Disease Research Center (IDRC). By 2010, the IDRC will provide nearly 25,000 sq. ft of total animal space to serve the needs of over 30 current principal investigators and a dozen collaborative institutions. The IDRC supports research on select agents, emerging pathogens, arboviruses, and mycobacterial diseases at Animal Biosafety Levels 2 and 3, and as such, is a unique facility in the Rocky Mountain region, and nationally. We propose here to increase autoclave capacity at the RMRBL cagewash facility to optimize biosafety and biosecurity sterilization of animal caging and equipment. Installation of a large pass-through autoclave is proposed in an area adjacent to the RMRBL animal facility that will also allow emergency redundancy sterilization in the case of failure of the primary decontaminating autoclave. The size and footprint of the proposed equipment is modeled after a highly functional autoclave located in another cagewash facility on campus, allowing equipment redundancy and proof of concept for the proposed machinery. Funding is also requested for movable animal caging to provide high-level containment housing for species other than mice, and transport housing for animal movement between holding areas and manipulation space. Altogether this proposal will fully equip the IDRC, allowing for projected growth of animal use, and the capacity to efficiently and effectively process equipment needed for specialized research performed at the site.