Rederivation and Internal Quarantine Suite (RIQS)

描述（由申请人提供）：本动物资源改进申请申请资金，用于翻新和装备加州大学欧文分校（UCI）现有的2332只ASF，以创建再分化和内部隔离套件（RIQS），以保护校园小鼠种群免受疾病侵害，从而支持phs资助的研究人员。RIQS设施将提供一个目前无法获得的专门空间，用于隔离和重新培育患病或可疑动物，通过将胚胎移植到干净的养母身上，产生无病的后代。RIQS功能包括：单独的隔离（脏）和再派生（干净）套件；通过气闸与建筑物其他部分隔离，包括通往“脏”套房的单独出入口；过滤进出空气；所有门上的卡码锁；组织通道：组织的通道；将房间压力从洁净区降至脏区；检查程序以防止人员在进入RIQS后进入干净的室内。RIQS将允许phs资助的研究人员安全地将有价值的基因工程小鼠菌株带到UCI进行再分化，这种做法在2006年因疾病而停止。RIQS允许使用专门的做法（例如屏障维护）来保护有价值的基因工程种畜，研究数据的质量，以及重要的校园和NCRR先前对动物设施空间的投资，特别是老鼠。一项新的ULAR政策将要求所有用于繁殖的基因工程小鼠重新衍生到新的屏障空间。这将维持对再衍生套件的需求，即使在校园所有目前的病原体怀疑小鼠都被重新衍生之后。RIQS提供了一个理想的地点和环境来培训员工的技能，从封堵和育种策略（动物技术人员）到胚胎移植和其他辅助生殖技术（高级和兽医技术人员）。

DESCRIPTION (provided by applicant): This animal resource improvement application requests funds to renovate and equip 2,332 ASF of existing vivarium and procedure space at the University of California, Irvine (UCI) to create the Rederivation & Internal Quarantine Suite (RIQS) to protect campus mouse populations from disease, thereby supporting PHS-funded researchers. The RIQS facility would provide a currently unavailable specialized space to isolate and rederive diseased or suspect animals to produce disease-free offspring via embryo transfer to clean foster mothers. RIQS features include: separate Quarantine (dirty) and Rederivation (clean) Suites; separation via airlock from other parts of the building, including separate entrance and exit doors to the "dirty" suite; filtration of both incoming and outgoing air; card coded locks on all doors; a pass-through for tissues; descending room pressurization from clean to dirty areas; carding procedures to prevent personnel from entering clean vivaria after entering the RIQS. RIQS will allow PHS-funded researchers to safely bring valuable strains of genetically engineered mice to UCI for rederivation, a practice stopped in 2006 due to disease. RIQS allows for the use of specialized practices (e.g. barrier maintenance) to protect valuable genetically engineered breeding stock, the quality of research data, and a significant campus and NCRR previous investment in animal facility space, especially for mice. A new ULAR policy will require all genetically engineered mice to be used for breeding be rederived into the new barrier space. This will sustain demand for the rederivation suite even after all current pathogen suspect mice on campus have been rederived. The RIQS offers an ideal location and environment to train staff in skills ranging from plugging and breeding strategies (animal technicians) to embryo transfers and other assisted reproductive technologies (senior and veterinary technicians).