Equipment for Gross Hall Vivarium

描述（由申请人提供）：本申请要求提供资金，以在一个新的设施，格罗斯霍尔动物资源中心（GHARC），由HHS CO 6 ARRA资助NIH资助号1C 06 RR 030131 -01，并在支持一组NIH资助的研究人员在加州大学欧文分校（UCI）举行的空间育种中心。该项目将实现UCI动物计划的具体目标，并解决目前预算中GHARC的缺陷。拟议的育种中心将包括两个饲养套房，每个40'x23' = 920平方英尺x2套房= 1840平方英尺，并将位于格罗斯霍尔动物资源中心（GHARC）的屏障内，占地17，433平方英尺（23，385总平方英尺），位于UCI校园最新的研究大楼Sue和Bill Gross Hall的地下室。育种中心将支持小鼠的育种和饲养，包括有价值的转基因小鼠。举个例子：为了更好地模拟阿尔茨海默病（AD）神经病理学，校长神经生物学和行为学教授Frank LaFerla创建了AD的三重转基因模型（3xTg-AD）。LaFerla没有将两个转基因（ssAPP和tau）杂交到独立的品系中，而是将两个转基因显微注射到来自纯合PS1 M146 V敲入小鼠的单细胞胚胎中，产生具有相同遗传背景的小鼠。这些小鼠被广泛用于AD研究，并出口到其他研究机构。在UCI，有价值的转基因小鼠育种者（如3xTg-AD）被安置在育种中心，采用严格的生物安全标准：广泛的病原体排除列表，强制性再衍生，有限的研究人员访问，适当的个人防护设备（PPE）等。申请的设备包括十二（12）个双侧笼架（140个笼/架）、四（4）个动物转移站和两（2）个2级A型生物安全柜。在UCI，只要有可能，就将有价值的转基因小鼠品系建立者安置在专门的育种中心--生物科学的McGaugh Hall和BRC的休伊特Hall。这些育种中心使用严格的生物安全标准：广泛的病原体排除清单，强制性重新衍生，有限的研究人员访问，以及适当的PPE。随着GHARC中7，280只小鼠笼的容量增加，休伊特霍尔的单个育种中心不足以为Gross-Gillespie-休伊特BRC复合体提供实验小鼠。GHARC的育种中心将支持18名研究人员，NIH的资金超过1080万美元，还有490万美元待定。笼子和设备将主要支持一组由PHS资助的基础和转化研究人员在再生医学，阿尔茨海默病，癌症，糖尿病和其他代谢紊乱，黄斑变性，色素性视网膜炎，疱疹相关失明，免疫学，脊髓损伤和中风中使用的种猪。GHARC和拟议的育种中心所在地格罗斯大厅已获得LEED白金认证，拟议的育种中心设施将使用“绿色”笼架（能效提高63%）;变风量（VAV）房间HVAC，校园内第一个动物园;和高效的干热灭菌器。

DESCRIPTION (provided by applicant): This application requests funds to equip a Breeding Center in holding space in a new facility, Gross Hall Animal Resource Center (GHARC), funded by an HHS CO6 ARRA grant NIH Grant no. 1C06RR030131-01 and in support of a group of NIH-funded researchers at the University of California, Irvine (UCI). The project would achieve specific aims for the UCI animal program, and addresses deficiencies in the GHARC as currently budgeted. The proposed Breeding Center would consist of two holding suites, each 40'x23' = 920 sf x 2 suites = 1840 sf total, and would be located inside the barrier in the Gross Hall Animal Resource Center (GHARC), which occupies 17,433 net assignable square feet (23,385 gross square feet) in the basement of Sue and Bill Gross Hall, the newest research building on the UCI campus. The Breeding Center will support breeding and holding of mice, including valuable genetically modified mice. One example: to better model Alzheimer's Disease (AD) neuropathology, Frank LaFerla, Chancellor's Professor of Neurobiology and Behavior, created a triple transgenic model of AD (3xTg-AD). Rather than crossing independent lines, LaFerla microinjected two transgenes (ssAPP and tau) into single- cell embryos from homozygous PS1M146V knockin mice, generating mice with the same genetic background. These mice are used extensively in AD research and exported to other research institutions. At UCI, valuable genetically altered mouse breeders such as the 3xTg-AD are housed in Breeding Centers using strict biosecurity standards: broad pathogen exclusion list, mandatory rederivation, limited research staff access, appropriate personal protective equipment (PPE), etc. The two Breeding Center holding/procedure suites consist of an Anteroom, Procedure room, and two Holding rooms. Requested equipment includes twelve (12) double sided cage racks (140 cages/rack), four (4) animal transfer stations, and two (2) Class 2 Type A biological safety cabinets. At UCI, valuable genetically altered mouse line founders are housed whenever possible in specialized Breeding Centers-McGaugh Hall in Biological Sciences and Hewitt Hall in the BRC. These Breeding Centers use strict biosecurity standards: broad pathogen exclusion list, mandatory rederivation, limited research staff access, and appropriate PPE. With the addition of capacity for 7,280 mouse cages in the GHARC, the single Breeding Center in Hewitt Hall is insufficient to provide experimental mice for the Gross-Gillespie-Hewitt BRC complex. The Breeding Center in the GHARC will support 18 researchers with NIH funding in excess of $10.8 million, and $4.9 million pending. The caging and equipment will support primarily breeding stock used by a group of PHS-funded basic and translational researchers in regenerative medicine, Alzheimer's disease, cancer, diabetes and other metabolic disorders, macular degeneration, retinitis pigmentosa, herpes-related blindness, immunology, spinal cord injury, and stroke. Gross Hall, site of the GHARC and proposed Breeding Center, has received LEED Platinum certification, and the proposed Breeding Center Facility would use "green" cage racks (63% more energy efficient); Variable Air Volume (VAV) room HVAC, the campus's first in a vivarium; and an efficient dry heat sterilizer.