Ultra Rapid Monitoring of Bacterial Nano-Growth and Antibiotic Susceptibility

描述(由调查人员提供)：细菌对抗生素的抗药性不断上升，被疾病控制和预防中心(CDC)称为“世界上最紧迫的健康问题之一”。由于细菌耐药性的增加，对快速抗生素敏感性测试的需求变得更加迫切。抗菌素耐药性的增加是一个跨越数十年和许多细菌菌株的普遍问题。在美国，每年200万的细菌感染中，大约有9万人死亡。像耐甲氧西林金黄色葡萄球菌(MRSA)这样的“超级细菌”现在杀死的美国人比艾滋病还多。因此，我们的长期目标是开发一种新型的设备，能够在1小时的时间尺度上进行快速的抗菌素耐药性测量，这比目前的方法要快得多。本应用的目的是建立一种用于细菌体外快速生长监测的新方法，用于快速评估抗生素敏感性。这项技术基于流体微阻力，使用了具有纳米级精度的新型磁性非线性振荡器，也可以进行“纳米生长”监测。这种方法将使医生和临床病理学家能够快速而自信地确定适当的抗菌治疗。首席研究员在将物理和化学技术应用于医疗应用方面具有相关经验，合作者是临床微生物学专家。该项目的具体目标是实现(1)一小群细菌和(2)单个细菌细胞的快速体外生长监测和快速抗生素敏感性测试。所描述的方法简单而廉价，最终将导致构建一种可以在任何临床环境中使用的设备。通过使用这种设备，将很快确定适当的抗菌疗法，这将减少抗菌素耐药性，最重要的是拯救生命。 与公共卫生相关：这项应用引入了一种具有纳米级灵敏度的新方法，用于非常快速地确定细菌对给定抗生素的敏感性。这项拟议的技术有望通过快速确定抗生素/药物的适当选择和剂量，极大地改进传染病的治疗。总体目标是通过一种快速有效的治疗方案减少患者的发病率和死亡率，这也将导致减少广谱抗生素的使用，从而减少具有抗生素耐药性的“超级细菌”的出现。

DESCRIPTION (provided by investigator): The rising resistance of bacteria to antibiotics has been referred to by the Centers for Disease Control and Prevention (CDC) as "one of the world's most pressing health problems." The need for rapid antibiotic susceptibility tests is becoming more urgent due to the increasing resistance of bacteria. Increased antimicrobial resistance is a pervasive problem that has spanned decades and many bacterial strains. In the US, out of 2 million bacterial infections each year, there are approximately 90,000 deaths. "Superbugs" like Methicillin resistant Staphylococcus aureus (MRSA) now kill more Americans than AIDS. Therefore, our long term goal is to develop a novel device that can perform rapid antimicrobial resistance measurements on the time-scale of 1 hour, which is much faster than current methods. The objective of this application is to develop a new method for rapid in vitro growth monitoring of bacteria for fast antibiotic susceptibility evaluations. The technique is based on fluidic micro-drag, using novel magnetic non- linear oscillators with nano-scale precision, which also allows "nano-growth" monitoring. This method will enable physicians and clinical pathologists to quickly and confidently determine the proper antimicrobial therapy. The Principal Investigator has relevant experience in applying physical and chemical techniques to medical applications and the collaborator is a clinical microbiology expert. The project's specific aims are to achieve rapid in vitro growth monitoring, as well as rapid antibiotic susceptibility testing, of (1) a small colony of bacteria and (2) single bacterial cells. The described approach, which is straightforward and inexpensive, will ultimately lead to the construction of a device that can be utilized in any clinical environment. Through the utilization of such a device, appropriate antimicrobial therapies will be quickly determined, which will reduce antimicrobial resistance and, most importantly, save lives. PUBLIC HEALTH RELEVANCE: This application introduces a new approach, with nano-scale sensitivity, for very rapid determination of bacterial susceptibility to a given antibiotic. The proposed technique is expected to dramatically improve the treatment of infectious diseases by enabling fast determination of the proper choice and dosage of antibiotics/medications. The overall goal is to reduce morbidity and mortality in patients through a quick and efficacious treatment regimen, which will also lead to a reduced use of broad spectrum antibiotics and thus to a reduced emergence of "superbugs" with antibiotics resistance.