RNA干扰-猫眼课题宝

RNA干扰

批准号：

7694421

负责人：

Jianzhu Chen

金额：

$42.79万

依托单位：

MASSACHUSETTS INSTITUTE OF TECHNOLOGY

依托单位国家：

美国

项目类别：

财政年份：

资助国家：

美国

项目状态：

未结题

起止时间：

至

来源：

https://reporter.nih.gov/project-details/7694421

关键词：

Address Affect Algorithms Animals Area Base Sequence Biological Assay Biological Process Biology Boston Cancer Center Cells Characteristics Chromatin Collaborations Collection Colorado Communities Complement Complex Computer Simulation Condition Contracts Cultured Cells DNA DNA Polymerase II DNA Polymerase III DNA Sequence DNA delivery Data Databases Development Ensure Fees Fostering Gene Expression Gene Silencing Gene Targeting Generations Genes Genetic Genetic Screening Genetic Transcription Genomics Goals Human In Vitro Individual Invasive Laboratories Lentivirus Vector Libraries Liver Luciferases Lung Malignant Neoplasms Mammalian Cell Mammals Mediating Mediation Medical Messenger RNA Methodology Methods MicroRNAs Molecular Genetics Mus New Jersey Nucleic Acid Regulatory Sequences Numbers Oligonucleotides Organ Participant Plasmids Polyethyleneimine Polymerase Polymerase Chain Reaction Polymers Procedures Process Production Proteins Protocols documentation Publishing RNA RNA Interference RNA Sequences Range Reagent Reporter Research Research Institute Research Personnel Research Project Grants Resources Retroviridae Role Selection Criteria Services Site Small Interfering RNA Small RNA Solutions Specificity Stem cells Structure Subfamily lentivirinae System Systems Biology Technology Testing Tetracycline Tetracyclines Time Tissues Training Transcription Initiation Transfection Transgenic Animals Validation Work Workplace animal tissue base cell type chemical synthesis cost cost effectiveness desire discount expression vector gain of function gene function high throughput screening improved in vivo interest loss of function member novel novel strategies plasmid DNA programs promoter repository research study response skills small hairpin RNA tool tumor progression vector web-accessible

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中文摘要

翻译

这一提议的一个关键方面，嵌入在三个研究项目中的每一个项目中，都是对参与癌症进展的特定细胞程序的计算模型所做预测的验证。这些预测需要在细胞和动物中通过功能获得或功能丧失实验进行测试。 RNA干扰（RNA interference，RNAi）是近年来在转录后水平调控基因表达的一种新的细胞机制，也是实验调控基因功能的有力工具。这些包括定义用于选择有效的小干扰RNA（siRNA）序列的改进的标准;用于递送短干扰RNA（siRNA）或表达短发夹RNA（shRNA）的改进的方法，所述短发夹RNA继而产生siRNA，从而使用逆转录病毒表达系统在哺乳动物细胞、组织和动物中稳定沉默基因。基于RNAi的集中和高通量筛选项目已经启动，以寻找参与基本生物过程以及与癌症相关的复杂病理条件的基因。ICBP RNAi资源将开发新的siRNA/shRNA递送技术，并将为计划在研究中使用RNAi技术的参与者提供支持。 RNAi资源设施的具体目标是： 1.生成并分发一组功能验证的siRNA和shRNA，这些siRNA和shRNA能够沉默ICBP感兴趣的基因。 2.开发和改进功能性siRNA序列的选择标准。 3.开发新的方法来实现siRNAs的组成型、调节型或组织特异性递送和shRNAs的表达。 4.为ICBP研究人员提供关于如何使用RNAi技术的技术和科学建议。

英文摘要

A key aspect of this proposal, embedded in each of the three research projects is the validation of predictions made by computational models of specific cellular programs involve in cancer progression. Those predictions need to be tested in cells and in animals by gain-of-function or loss of-function experiments. RNA interference (RNAi) has emerged as a novel cellular mechanism regulating gene expression at the posttranscriptional level and as a powerful tool to control gene function experimentally There have been several recent advances in the biology and application of RNAi. These include the definition of improved criteria for selecting effective small interfering RNA (siRNA) sequences ; improved methods for delivery of short interfering RNAs (siRNAs) or expression of short hairpin RNAs (shRNAs), which in turn generate siRNAs leading to stable silencing of genes in mammalian cells, tissues, and animals using retroviral expression systems. Both focused and high-throughput screening projects based on RNAi have been initiated to search for genes involved in basic biological processes, as well as in complex pathological conditions associated with cancer. The ICBP RNAi resource will develop new siRNA/shRNA delivery technologies and will provide support to the participants in this program that plan to use RNAi technologies in their research. The specific aims of the RNAi resource facility are to: 1. Generate and distribute a set of functionally validated siRNAs and shRNAs that are able to silence genes of interest to the ICBP. 2. Develop and improve selection criteria for functional siRNA sequences. 3. Develop new approaches to achieve constitutive, regulated, or tissue-specific delivery of siRNAs and expression of shRNAs. 4. Provide technical and scientific advice to ICBP investigators on how to use RNAi technologies.

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