Behavioral actions of angiotensin II: Circuits and intracellular signals

项目概要：候选人、环境和研究。本提案描述了一项为期5年的培训 为候选人提供实现其长期目标所需的专业知识的计划 作为一名独立的行为神经科学家， 大学候选人获得了博士学位。自2001年以来，他一直在进行研究， 动物生物学系和马奥尼神经学研究所博士后研究员 宾夕法尼亚大学的自然科学系。马奥尼神经科学研究所 拥有来自18个系和6个学院的180多名教职员工 并为候选人提供理想的培训环境。拟议 导师，史蒂芬·J·弗卢哈蒂博士，是行为领域的一位知名研究者。 神经科学和细胞和行为对激素反应的专家， 建议的实验，有效的dipsogen血管紧张素II（AngII）。研究计划的重点是 水、盐和食物摄入量，体液、心血管和能量的关键成分 体内平衡将这些行为理解为独立但相关的摄食行为 有可能揭示他们的控制和监管的新见解。实验 本文所述的是旨在确定与激素相关的细胞内事件 刺激摄食行为。AngII受体产生多种细胞内反应， 然而，任何这些反应和治疗所产生的行为之间的联系， Angl仍然不清楚。这里描述的实验利用了新的药理学方法。 方法来确定这些不同的细胞内反应的行为相关性， angll诱导的摄食行为，并探讨一般摄食行为的共性。 项目摘要：与公共卫生的相关性。水、盐和食物的摄入对于 维持体内平衡。影响体液、心血管和能量的疾病 体内平衡包括高血压、心脏或肾脏疾病、糖尿病和肥胖症。确定 激素介导这些行为的神经机制对于一个完整的 了解的现象，并可能导致新的治疗方法，以打击 相关疾病状态。

Project summary: candidate, environment, and research. This proposal describes a 5-year training program designed to provide the candidate with the expertise needed to achieve his long-term goal of developing a career as an independent behavioral neuroscientist at an academic research university. The candidate received a Ph.D. in 2001 and has since been conducting research as a postdoctoral fellow in the Department of Animal Biology and the Mahoney Institute for Neurological Sciences at the University of Pennsylvania. The Mahoney Institute for Neurological Sciences houses over 180 faculty members from eighteen departments and six schools within the University of Pennsylvania and provides an ideal training environment for the candidate. The proposed mentor, Dr. Steven J. Fluharty, is a well-established researcher in the field of behavioral neuroscience and an expert in the cellular and behavioral responses to the hormone studied in the proposed experiments, the potent dipsogen angiotensin II (Angll). The research plan focuses on water, salt, and food intake, key components of body fluid, cardiovascular, and energy homeostasis. Understanding these behaviors as separate, but related forms of ingestive behavior has the potential to reveal novel insights into their control and regulation. The experiments described here are designed to determine the intracellular events associated with the hormonal stimuli for ingestive behavior. Angll receptors produce a diverse array of intracellular responses, yet the connection between any of these responses and the behaviors produced by treatment with Angll remains unclear. The experiments described here take advantage of novel pharmacological approaches to determine the behavioral relevance of these divergent intracellular responses to Angll-induced ingestive behaviors and explore commonalities of ingestive behaviors in general. Project summary: Relevance to public health. Ingestion of water, salt, and food is critical for maintenance of homeostasis. Disorders affecting body fluid, cardiovascular, and energy homeostasis include hypertension, heart or kidney disease, diabetes, and obesity. Determining the neural mechanisms through which hormones mediate these behaviors is critical for a complete understanding of the phenomena and may lead to novel therapeutic approaches to combat the relevant disease states.