DEVELOPMENT OF CORE SERVICE FOR CELLULAR IMMUNE ASSAYS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 细胞免疫学核心应要求为内部和外部研究人员的特定研究项目提供免疫学服务。目前的服务包括在最佳检测分析的标准化程序下的样品制备、ELISPOT检测的规划和执行、数据处理和演示、细胞内细胞因子染色，以及在这些检测中用作抗原刺激剂的重组牛痘病毒库存的制备。我们将继续使用Mabtech Elispot系统以及U-Cytech系统，具体取决于特定的项目和合作者。在很大程度上，我们继续使用Zellnet咨询服务来记录和分析Elispot的结果。细胞免疫学核心还为研究人员提供MHC分型服务。在这一年里，TNPRC分配给不同项目的200多只恒河猴接受了一种特定的恒河猴MHC-I和II基因的检测。今年，CORE还向调查人员提供了Rheus MHC I类四聚体，进行了400多次检测。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cellular Immunology Core provides immunology services to specific research projects of in-house and outside investigators as requested. Current services include sample preparation under standardized procedures for optimal assay analysis, planning and performance of ELISPOT assays, data processing and presentation, intracellular cytokine staining, and preparation of recombinant vaccinia viral stocks for use as antigenic stimulants in these assays. We are continuing to use the MabTech ELISPOT system as well as the U-Cytech system depending on the particular project and collaborators. For the most part we have continued to use the Zellnet Consulting service for recording and analyzing the ELISPOT results. The Cellular Immunology Core also provides MHC typing service to investigators. During this year, more than 200 Rhesus monkeys assigned to different projects at the TNPRC were tested for the presence of a particular Rhesus monkey MHC-I and II genes. The core has also provided Rhesus MHC class I tetramers for over 400 assays to investigators this year.