CELLS AND TISSUES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 描述 分子组织学实验室负责人约翰·帕特里克森 这一核心单位成立于2007年，由约翰·帕特里克森博士领导。为MSM研究人员、研究生和亚特兰大大学中心提供组织学服务。该设备可提供基本的组织学服务，包括：石蜡包埋、石蜡切片、冰冻切片、免疫组织化学和H&E染色以及原位杂交。该设施包括石蜡组织包埋中心、旋转显微切割机、低温恒温器、组织漂浮浴、微波炉、组织处理机和载玻片加温器。 电子显微镜实验室-领队罗宇权 RCMI EM核心设施成立于1985年，提供完整的扫描和传输服务，从标本固定/脱水/临界点干燥/嵌入/聚合/阻断trimming/sectioning/slide-making/formvar-coating，到使用JELOL820扫描和JEOL1200EX透射电子显微镜检查和记录准备好的样品。 影像实验室-姚秀宝，领导 图像分析核心(IAF)目前由4台主要设备组成：一台新购置的DeltaVision核心去卷积显微镜、一台徕卡TCS SP5共焦显微镜、一台能够测量荧光、发光和吸光度的Tecan平板阅读器，以及一台蔡司Axioplan直立式荧光显微镜，配有Spot Insight数码相机和软件。该设施中还放置了2个孵化器(1个CO2，水套)、一个细胞培养罩和一台蔡司Axiovert 25细胞培养显微镜，以帮助在IAF进行实验时维护细胞。该设施的监督由IAF主任、系教授姚学标博士提供。生理学博士(5%)和实验室协调员Andrew Shaw先生(90%)。 服务费： 分子组织学实验室 研究人员负责固定和阻断组织。对于常规固定，建议用10%中性缓冲福尔马林或多聚甲醛的新鲜溶液固定组织。为获得最佳效果，建议少量购买固定剂。(详情请联系实验室) 如需冰冻组织制剂，请联系实验室。 控罪摘要： 石蜡：包埋5美元； 切割、染色、封面幻灯片-第一张幻灯片4.00美元； 来自同一块的额外染色幻灯片$2.00，未染色的幻灯片$1.00 冰冻：未染色切片：3：00/张幻灯片，同一张幻灯片上的切片$1.00/切片浮动冻结切片：贴装和封套幻灯片$2.00/张 用户自己裁剪自己的部分：每小时20美元 电子显微镜实验室 每份样品制备150.00美元，每张透射电子显微镜底片2.5美元，每张扫描电子显微镜偏光片5美元。这包括： A)样品的完整准备(即固定、脱水、临界点干燥、溅射涂层、包埋、聚合、刀具制作、修整、H/E 幻灯片准备、切片、Forvar涂层网格准备和随后的网格染色...) B)利用透射和/或扫描电子显微镜对准备好的样品进行检查和记录。 C)将EM底片显影并扫描到CD上供调查人员使用。 产生的收入包括基本的EM材料、化学品、EM胶片底片和一般用品的成本、更换钻石刀(3000.00美元)、设备维修和维护(例如。临界点烘干机大修，1500.00美元；超微切割机服务，1500.00美元)和小型设备采购 成像实验室 反卷积显微镜，每小时40美元。 共焦显微镜：收费40美元/小时，无人值守30美元/小时。用户必须熟练使用设备，然后才能允许无人值守使用。 泰肯平板阅读器：20美元/小时。平均运行时间约为5分钟；然而，时间进程实验将在设备保留的整个时间内付费。 蔡司立式荧光显微镜：20美元/小时。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION Molecular Histology Lab  John Patrickson, Leader This core unit was established in 2007 under the leadership of Dr. John Patrickson. Provides histology services to the MSM research faculty, graduate students, and the Atlanta University Center. This facility is equipped to provide basic histological services including: paraffin embedding, paraffin sections, frozen sections, immunohistochemical and H&E staining, and in situ hybridization. The facility consists of a paraffin tissue embedding center, rotary microtomes, cryostat, tissue floatation bath, microwave, tissue processor, and slide warmers. Electron Microscopy Lab - Woo-Kuen Lo, Leader Established in 1985, the RCMI EM Core facility provides complete scanning and transmission services, from specimen fixation/dehydration/critical point drying/ embedding/polymerization/ block trimming/sectioning/slide-making/formvar-coating, to examination and documentation of prepared samples utilizing JEOL 820 scanning and JEOL 1200EX transmission electron microscopes. Imaging Lab - Xieubao Yao, Leader The Image Analysis Core (IAF) presently consists 4 primary pieces of equipment: a newly acquired DeltaVision Core deconvolution microscope, a Leica TCS SP5 confocal microscope, a Tecan plate reader capable of fluorescent, luminescent and absorbance measurements, and a Zeiss Axioplan upright fluorescent microscope with attached Spot Insight digital camera and software. Also housed in the facility are 2 incubators (1 CO2 , water jacketed), a cell culture hood and a Zeiss Axiovert 25 cell culture microscope to aid in the maintenance of cells while performing experiments in the IAF. Oversight of the facility is provided by Dr. Xuebiao Yao, Director of the IAF and Professor in the Dept. of Physiology (5%) and Mr. Andrew Shaw, Lab coordinator (90%). Service Charges: Molecular Histology Lab The investigator is responsible for the fixation and blocking of the tissue. For routine fixation it is recommended that the tissue is fixed with a fresh solution of 10% neutral buffered formalin or paraformaldehyde. For best results, it is recommended that the fixatives be purchased in small quantities. (Contact the laboratory for details) For frozen tissue preparations contact the laboratory. Summary of Charges: Paraffin: Embedding $5.00; Cut, Stain, Coverslip -1st slide $4.00; Additional stained slide from same block $2.00, unstained $1.00 Frozen: Unstained section: 3:00/slide, section on same slide $1.00/section Floating frozen section: mounted & coverslip $2.00/slide User cuts their own cut their own sections: $20.00 per hour Electron Microscopy Lab $150.00 per sample preparation, $2.50 per TEM negative, $5.00 per SEM polaroid. This includes: a) Complete preparation of samples (i.e. fixation, dehydration, critical point drying, sputter coating, embedding, polymerization, knife-making, trimming, H/E slide-preparation, sectioning, formvar-coated grid preparation and subsequent grid-staining...) b) Examination and documentation of prepared specimens utilizing Transmission and/or Scanning Electron Microscope. c) EM negatives developed and scanned onto CD for investigator. The generated revenue covers cost of basic EM materials, chemicals, EM film negatives and general supplies, diamond knife replacement ($3000.00), equipment repairs and maintenance (eg. critical point dryer overhaul, $1500.00; Ultramicrotome service, $1500.00) and small equipment purchase Imaging Lab Deconvolution microscopy $40 / hr. Confocal microscopy: $40 / hr attended; $30 / hr unattended. User must demonstrate proficiency with the equipment before unattended use will be allowed. Tecan Plate Reader: $20 / hr. Average run takes approximately 5 min.; however, time-course experiments will be billed for the entire time that the equipment will be reserved. Zeiss upright fluorescent microscope: $20 / hr.