BTSL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 多萝西娅·V·帕克，领袖 莫尔豪斯医学院的生物医学技术服务实验室(BTSL)已经存在了20年。在此期间，BTSL极大地加强和支持了该机构基础和临床科学系的生物医学研究。这种支持延伸到他们实验室的人员以及医学院和邻近的AUC机构的研究生和本科生。BTSL是该机构最大的核心设施，占据了主校区的八个实验室地点。它为所有类型的多用途设备、研究必要性、技术、团队以及一对一仪器指令和协议开发提供一致的支持。 BTSL目前还提供克隆和多克隆抗体生产的专业服务。克隆抗体是用Balb/c小鼠产生的。多克隆抗体是用新西兰大白兔生产的。多克隆和单克隆核心制造针对结合的合成肽、可溶性蛋白、0趋化因子、细胞因子、细胞抗原、从聚丙烯酰胺凝胶中提取的蛋白条带以及各种其他抗原的抗体。 细胞计数和细胞分类功能被重新置于BTSL之下。负责细胞计数和细胞分类功能的技术人员乌代·辛格博士于2007年底/2008年初离开了学校。此外，这些非常陈旧的设备现在基本上已经坏了。 服务费： 服务费与去年报告中列出的相同。我们目前正在评估和考虑新的收费金额。 1)液氮每升0.85美元 2)单抗生产$1,500.00(包括动物成本、接种、融合和克隆)外部研究人员的价格为$2,000.00。 3)多克隆抗体生产费用按兔只数、免疫次数、出血量和所需血清量计算。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dorothea V. Parker, Leader The Biomedical Technology Service Laboratory (BTSL) at Morehouse School of Medicine has been in existence for twenty years. During this time, the BTSL has enhanced and supported the biomedical research of the basic and clinical science faculty at the institution tremendously. This support extends to the personnel in their laboratories as well as graduate and undergraduate students from the medical school and neighboring AUC institutions. The BTSL is the largest core facility at the institution occupying eight laboratory locations on the main campus. It provides consistent support for all types of multi-use equipment, research necessities, techniques, group as well as one on one instrumentation instruction and protocol development. The BTSL also currently offers the specialized services of monoclonal and polyclonal antibody production. Monoclonal antibodies are produced using Balb/c mice. Polyclonal antibodies are produced using New Zealand white rabbits. The polyclonal and monoclonal core makes antibodies against conjugated synthetic peptides, soluble proteins,0 chemokines, cytokines, cellular antigens, protein bands excised from polyacrylamide gels plus a variety of other antigens. The Cytometry and Cell Sorting Function was placed back under the BTSL. The technician, Dr Udai Singh, for the Cytometry and Cell Sorting Function left the school in late 2007/early 2008. Additionally, the equipment, which was very old is now essentially down. Service Charges: Service charges have remained the same as those listed in last year's report. We are currently assessing and considering new charge amounts. 1) Liquid Nitrogen $0.85/liter 2)Monoclonal Antibody Production $1,500.00 (includes animal costs, inoculations, fusion and cloning) The price for outside investigators is $2,000.00. 3) Polyclonal Antibody Production charges are based on the number of rabbits, immunizations, bleeds and the serum amount required.