NIR Instrument for Singlet Oxygen Mapping during PDT

描述（由申请人提供）：该提案的重点是光动力治疗（PDT）的剂量测定领域，特别是乳腺癌治疗。这是至关重要的三个PDT组件的最佳剂量-光敏剂，光通量和氧气-参与治疗的成功结果。虽然剂量不足可能导致癌症复发，但剂量过量可能导致不希望的健康组织损伤。一种广泛接受的精确剂量测定方法是基于这样一个事实，即PDT过程中局部产生的细胞毒性新生氧的量与治疗结果直接相关。使用近红外光的漫射光学断层扫描（DOT）已成功用于乳腺肿瘤的功能成像，其基于与健康组织相比的内源性和外源性光谱对比度。我们将使用这种成熟的DOT技术来基于光通量下光敏剂的发光来执行新生氧浓度的实时空间映射。在第一阶段，我们将开发一种检测方法，以证明光敏剂的时间依赖性发光特性与PDT结果直接相关。基于第一阶段的结果，我们将在第二阶段开发空间新生氧成像模块的原型，该原型将在冷却的多探测器阵列上进行并行采集，以实现高分辨率和高通量成像。低成本，紧凑的PDT剂量成像模块将被设计为与现有的DOT系统兼容，以扩展其在乳腺癌和其他癌症治疗的PDT期间的监测应用。 公共卫生相关性：基于光动力学疗法（PDT）中使用的药物的时域特性，扩散发光成像可用于获得新生氧浓度的定量空间图。乳腺肿块切除术后PDT预防癌症局部复发是主要的目标应用，目的是改善治疗结果并最大限度地减少副作用。其他应用可能包括在脑癌、头颈癌和其他非癌性疾病的PDT治疗期间监测剂量评估。

DESCRIPTION (provided by applicant): The focus of the proposal is in the area of dosimetry for photodynamic therapy (PDT), especially for breast cancer treatment. It is crucial that the optimal dosage of the three PDT components - photosensitizer, light fluence, and oxygen - be involved for the successful outcome of the treatment. While under dosing can lead to recurrence of the cancer, over dosing can lead to undesirable healthy-tissue damage. A widely accepted method for accurate dosimetry is based on the fact that the quantity of cytotoxic nascent oxygen generated locally during PDT is directly correlated to the treatment outcome. Diffuse optical tomography (DOT) using near infrared light has been used successfully for functional imaging of breast tumors based on their endogenous and exogenous spectral contrast compared to healthy tissue. We will use this well- established DOT technique to perform real-time spatial mapping of nascent oxygen concentration based on the luminescence from the photosensitizer under light fluence. During Phase I, we will develop a detection methodology to demonstrate that the photosensitizer time dependent luminescence characteristics are directly correlated to PDT outcome. Based on the results of Phase I, we will develop a prototype of the spatial nascent oxygen imaging module during Phase II, which will have parallel acquisition across a cooled multi-detector array for high resolution and high throughput imaging. The low-cost, compact PDT dosimetric imager module will be designed to be compatible with existing DOT systems to extend their applications for monitoring during PDT for breast and other cancer treatments. PUBLIC HEALTH RELEVANCE: Diffuse luminescence imaging can be used to obtain quantitative spatial maps of nascent oxygen concentration based on time domain characteristics of the drugs used in photodynamic therapy (PDT). PDT following breast lumpectomy to prevent local recurrence of cancer is the primary target application, with the goal of improving treatment outcome and minimizing side effects. Other applications could include monitoring for dose evaluation during PDT treatments in brain cancers, head and neck cancers, and other non-cancerous conditions.