ADIPOSITY, ACTIVITY AND ASTHMA (AAA) A CLINICAL TRANSLATIONAL STUDY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项研究将在哮喘患者和健康受试者中，一方面确定肥胖的解剖学和生化指标，另一方面确定呼吸道炎症和氧化应激指标之间的关系。肥胖的解剖学测量包括全身脂肪、皮下脂肪和内脏脂肪的测量。肥胖症的生化指标包括血清瘦素(一种促炎症脂肪因子)和脂联素(一种抗炎症脂肪因子)水平的比率。呼吸道炎症指标包括呼气冷凝液(EBC)中的肿瘤坏死因子-a水平和诱导痰(IS)中的嗜酸性粒细胞阳离子蛋白水平。呼吸道氧化应激指标包括外周血和血浆中8-异前列腺素水平。 在哮喘患者和健康受试者中，肥胖与呼吸道炎症/氧化应激之间的关系是不同的。 确定哮喘状态和肥胖在呼吸道炎症/氧化应激中是否存在交互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study will determine the relationship between anatomical and biochemical measures of adiposity on one hand, and markers of airway inflammation and oxidant stress on the other hand, in asthmatics and in healthy subjects. Anatomical measures of adiposity include measures of total body fat, subcutaneous and visceral fat. Biochemical measures of adiposity include the ratio of the levels of serum leptin (a pro-inflammatory adipokine) and adiponectin (an anti-uinflammatory adipokine). Measures of airway inflammation include levels of TNF-a in exhaled breath condensate (EBC) and eosinophil cationic protein level in induced sputum (IS). Measures of airway oxidant stress includ 8-isoprostane levels in EBC and in plasma. The association between adiposity and airway inflammation/oxidant stress is different between asthmatics and healthy subjects. Determine if there is an interaction between asthma status and adiposity on airway inflammation/oxidant stress.