PROFILING PROTEIN INTERACTIONS OF H33, H32, AND H31

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们使用我们的方法研究了H3蛋白，以快速有效地分离GFP标记的蛋白及其相互作用伙伴。我们最初的实验集中在H3.3变异体上，并通过GFP标签优化了该蛋白的裂解和分离条件。 研究结果发表于： 不同的因素控制组蛋白变异体H3.3在特定基因组区域的定位。 戈德堡AD，巴纳辛斯基LA，卢凯姆，刘易斯PW，埃尔塞塞SJ，斯塔德勒S，德威尔S，劳M，郭X，李X，文D，Chapgier A，Dekelver RC，Miller JC，Lee YL，Boydston EA，Holmes MC，Gregory PD，GrealJM，Rafii S，杨C，Scambler PJ，Garrick D，Gibbons RJ，Higgs DR，Cristea IM，Urnov FD，郑D，Allis CD。 牢房。2010年3月5日；140(5)：678-91。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We studied the H3 proteins using our approach for the rapid and efficient isolation of a GFP-tagged protein with its interacting partners. Our first experiments focused on the H3.3 variant, and we optimized the lysis and isolation conditions for the purification of this protein via a GFP tag. The results were published: Distinct factors control histone variant H3.3 localization at specific genomic regions. Goldberg AD, Banaszynski LA, Noh KM, Lewis PW, Elsaesser SJ, Stadler S, Dewell S, Law M, Guo X, Li X, Wen D, Chapgier A, DeKelver RC, Miller JC, Lee YL, Boydston EA, Holmes MC, Gregory PD, Greally JM, Rafii S, Yang C, Scambler PJ, Garrick D, Gibbons RJ, Higgs DR, Cristea IM, Urnov FD, Zheng D, Allis CD. Cell. 2010 Mar 5;140(5):678-91.