GENOME-WIDE VIEW OF REPLICATION FORK PROGRESSION AND ARREST

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 真核生物中的DNA复制已经在芽殖酵母的全基因组水平上被广泛研究。这些研究已经导致了这样一幅图景，其中DNA复制从大量的起点开始，并在基因组的不同部分以广泛可变的速率进行。 进行叉也被描述为在野生型酵母基因组中的许多特定位点处暂停。这些数据已被解释为意味着复制叉进展的动力学受到局部染色质结构或架构的强烈影响。在这里，我们通过细胞周期监测复制叉偶联GINS复合物的基因组关联。描述这项工作的手稿已经出版： M.D. Sekedat，D. Feny？，R.S.放大图片作者：A. Aitchison，B.T. Chait“GINS Motion Reveals Replication Fork Progression is Remarkably Uniform Throughout the Yeast Genome”Mol Systems Biol，6（2010）353 我们目前正在将这项工作扩展到对DNA聚合酶的动力学的研究

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DNA replication in eukaryotes has been extensively studied at the genome wide level in budding yeast. These studies have led to a picture wherein DNA replication initiates from a large number of origins and progresses at widely variable rates over different parts of the genome. The progressing fork has also been described to pause at numerous specific sites in the genome of wild type yeast. These data have been interpreted to mean that the dynamics of replication fork progression are strongly affected by local chromatin structure or architecture. Here, we monitored the genomic association of the replication fork-coupled GINS complex through the cell cycle. A manuscript describing this work has been published: M.D. Sekedat, D. Feny¿, R.S. Rogers, A.J. Tackett, J.D. Aitchison, B.T. Chait "GINS Motion Reveals Replication Fork Progression is Remarkably Uniform Throughout the Yeast Genome" Mol Systems Biol, 6 (2010) 353 We are currently extending this work to the study of the dynamics of the epsilon and delta DNA polymerases